Volm M, Efferth T, Mattern J
Institute of Experimental Pathology, German Cancer Research Center, Heidelberg.
Arzneimittelforschung. 1989 Aug;39(8):828-31.
The development of resistance to vincristine and dactinomycin has been investigated in a human epidermoid lung xenograft line grown in nude mice. With 1 mg/kg BW vincristine and 0.5 mg/kg BW dactinomycin per passage, resistance of the solid tumors was visible already at the second transplantation. The vincristine-resistant subline showed cross-resistance to dactinomycin and doxorubicin, and the dactinomycin-resistant subline only to vincristine. To determine whether multidrug resistance genes were overexpressed in the resistant sublines, slot blots were performed using the cDNA probe pcDR 1.5. Slightly elevated RNA levels could be detected in the vincristine-resistant subline and in the dactinomycin-resistant subline.
在裸鼠体内生长的人肺表皮样癌异种移植瘤模型中，研究了对长春新碱和放线菌素D耐药性的发展情况。每次传代时给予1mg/kg体重的长春新碱和0.5mg/kg体重的放线菌素D，在第二次移植时即可观察到实体瘤产生耐药性。长春新碱耐药亚系对放线菌素D和阿霉素表现出交叉耐药性，而放线菌素D耐药亚系仅对长春新碱有交叉耐药性。为了确定多药耐药基因在耐药亚系中是否过度表达，使用cDNA探针pcDR 1.5进行了狭缝印迹分析。在长春新碱耐药亚系和放线菌素D耐药亚系中均可检测到RNA水平略有升高。
