使用分离染色质片段蛋白质组学方法对染色质位点进行蛋白质组表征

Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach.

作者信息

Kan Sophie L, Saksouk Nehmé, Déjardin Jérome

机构信息

INSERM AVENIR, Institute of Human Genetics CNRS UPR1142, 141 rue de la Cardonille, 34000, Montpellier, France.

出版信息

Methods Mol Biol. 2017;1550:19-33. doi: 10.1007/978-1-4939-6747-6_3.

DOI:10.1007/978-1-4939-6747-6_3

PMID:28188520

Abstract

The biological functions of given genomic regions are ruled by the local chromatin composition. The Proteomics of Isolated Chromatin segments approach (PICh) is a powerful and unbiased method to analyze the composition of chosen chromatin segments, provided they are abundant (repeated) or that the organism studied has a small genome. PICh can be used to identify novel and unexpected regulatory factors, or when combined with quantitative mass spectrometric approaches, to characterize the function of a defined factor at the chosen locus, by quantifying composition changes at the locus upon removal/addition of that factor.

摘要

特定基因组区域的生物学功能由局部染色质组成决定。分离染色质片段蛋白质组学方法（PICh）是一种强大且无偏倚的方法，用于分析所选染色质片段的组成，前提是它们丰富（重复）或者所研究的生物体基因组较小。PICh可用于识别新的和意想不到的调控因子，或者与定量质谱方法结合使用，通过在去除/添加该因子后对所选位点的组成变化进行定量，来表征特定因子在所选位点的功能。