Department of Immunology, Microbiology and Physiological Chemistry, West Pomeranian University of Technology in Szczecin, al. Piastów 45, 70-311 Szczecin, Poland.
Institute of Chemical Engineering and Environmental Protection Processes, West Pomeranian University of Technology in Szczecin, al. Piastów 42, 71-065 Szczecin, Poland.
Carbohydr Polym. 2017 Apr 1;161:208-218. doi: 10.1016/j.carbpol.2016.12.063. Epub 2016 Dec 28.
The main aim of the current study was to evaluate the usefulness of the two-dimensional correlation analysis (2DCorr) technique in assessing the physicochemical properties of bacterial cellulose (BC) depending on the synthesis time and Komagataeibacter xylinus strain used for its production. It was demonstrated that 2DCorr analysis allows to characterize substantial changes in the structure of BC at the molecular level occurring during its synthesis, which are difficult to determine using ATR-FTIR spectral analysis. Furthermore, it was found that 2DCorr analysis allows to identify the differences in the structure and the properties of BC according to the bacterial strain used for its synthesis. It was concluded that 2DCorr analysis of the ATR-FTIR spectra may be a useful tool for controlling BC production in order to obtain material with the desired properties.
本研究的主要目的是评估二维相关分析（2DCorr）技术在评估根据合成时间和用于生产的木醋杆菌菌株的细菌纤维素（BC）的物理化学性质方面的有用性。结果表明，2DCorr 分析允许在分子水平上表征在其合成过程中发生的 BC 结构的实质性变化，这很难使用 ATR-FTIR 光谱分析来确定。此外，还发现 2DCorr 分析可以根据用于合成 BC 的细菌菌株来识别 BC 的结构和性质的差异。结论认为，ATR-FTIR 光谱的 2DCorr 分析可能是控制 BC 生产的有用工具，以获得具有所需性质的材料。
