Lawler M, McCann S R, Conneally E, Humphries P
Department of Genetics, Trinity College Dublin, Ireland.
Br J Haematol. 1989 Oct;73(2):205-10. doi: 10.1111/j.1365-2141.1989.tb00253.x.
Chimaerism was assessed in five recipients following sex mismatched allogeneic bone marrow transplantation. Techniques included karyotyping of bone marrow cells, dot blot DNA analysis of blood and bone marrow suspensions, and in vitro amplification of DNA by the polymerase chain reaction (PCR) using blood and bone marrow suspensions and stored bone marrow slides. Results of karyotypic analysis suggested complete chimaerism in four patients, while in one patient mixed chimaerism was detected. Mixed chimaerism was also detected, however, in a second patient using PCR and confirmed by dot blot analysis on all tissues examined. PCR is a sensitive tool for investigation of chimaerism following bone marrow transplantation. Since this technique does not require radioactivity, it is an attractive method for use in a clinical laboratory. This technique represents a further development in the use of DNA methodologies in the assessment of haematological disease.
对5例接受性别不匹配的异基因骨髓移植的受者进行了嵌合体评估。技术包括骨髓细胞的核型分析、血液和骨髓悬液的斑点印迹DNA分析，以及使用血液和骨髓悬液及储存的骨髓玻片通过聚合酶链反应（PCR）进行DNA体外扩增。核型分析结果显示4例患者为完全嵌合体，而1例患者检测到混合嵌合体。然而，在第二例患者中也使用PCR检测到了混合嵌合体，并通过对所有检查组织的斑点印迹分析得到证实。PCR是骨髓移植后嵌合体研究的一种敏感工具。由于该技术不需要放射性，因此是临床实验室中一种有吸引力的方法。该技术代表了DNA方法在血液系统疾病评估中的进一步发展。
