Yang Qiong, Chu Qing, Zhao Xueyan, Xu Tianjun
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Fish Shellfish Immunol. 2017 Apr;63:165-172. doi: 10.1016/j.fsi.2017.02.005. Epub 2017 Feb 8.
Interleukin (IL)-1β is a prototypical proinflammatory cytokine that belongs to the IL-1 family. This cytokine possesses two receptor types, namely, IL-1 receptor type I (IL-1RI) and IL-1 receptor type II (IL-1RII). IL-1RI, is an IL-1 receptor that plays a crucial role in immune responses and IL-1RII is a decoy receptor for IL-1β signaling inhibitors in mammals. IL-1β, together with its two types of receptors, has been characterized in mammals and implicated in immunity. However, IL-1β and IL-1 receptors in teleost species have been rarely investigated. In this study three genes, namely, IL-1β, IL-1RI, and IL-1RII, were identified and characterized from miiuy croaker. Structural and comparative analysis revealed that miiuy croaker IL-1β, IL-1RI and IL-1RII, particularly their functional domains, were conservative in most of the species. Simultaneously, synteny phylogenetic analysis indicated that IL-1β and IL-18 were widely distributed in vertebrates and hence might be the ancestors of the IL-1 family. Challenge experiment demonstrated that IL-1β, IL-1RI and IL-1RII expression in miiuy croaker was induced by LPS and poly (I:C). IL-1RI expression was also induced by the overexpressed miiuy croaker IL-1β protein which in cell supernatant, whereas IL-1RII was not induced.
白细胞介素（IL）-1β是一种典型的促炎细胞因子，属于IL-1家族。这种细胞因子有两种受体类型，即IL-1受体I型（IL-1RI）和IL-1受体II型（IL-1RII）。IL-1RI是一种在免疫反应中起关键作用的IL-1受体，而IL-1RII是哺乳动物中IL-1β信号抑制剂的诱饵受体。IL-1β及其两种受体类型在哺乳动物中已得到表征，并与免疫有关。然而，硬骨鱼类中的IL-1β和IL-1受体很少被研究。在本研究中，从真鲷中鉴定并表征了三个基因，即IL-1β、IL-1RI和IL-1RII。结构和比较分析表明，真鲷的IL-1β、IL-1RI和IL-1RII，特别是它们的功能结构域，在大多数物种中是保守的。同时，共线性系统发育分析表明，IL-1β和IL-18在脊椎动物中广泛分布，因此可能是IL-1家族的祖先。攻毒实验表明，真鲷中的IL-1β、IL-1RI和IL-1RII表达受脂多糖（LPS）和聚肌苷酸胞苷酸（poly (I:C)）诱导。细胞上清液中过表达的真鲷IL-1β蛋白也能诱导IL-1RI表达，而IL-1RII未被诱导。
