Agnello Stefano, Gasperini Luca, Mano João F, Pitarresi Giovanna, Palumbo Fabio S, Reis Rui L, Giammona Gaetano
Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Taipas, Guimarães, Portugal.
Int J Biol Macromol. 2017 May;98:646-653. doi: 10.1016/j.ijbiomac.2017.02.029. Epub 2017 Feb 9.
New derivatives of gellan gum (GG) were prepared by covalent attachment of octadecylamine (C-NH) to polysaccharide backbone via amide linkage by using bis(4-nitrophenyl) carbonate (4-NPBC) as a coupling agent. The effect of the alkyl chain grafted onto hydrophilic backbone of high molecular weight GG was investigated in terms of physicochemical properties and ability of new derivatives to form hydrogels. A series of hydrogels was obtained in solutions with different kind and concentration of ions and their stability and mechanical properties were evaluated. The obtained derivatives resulted soluble at temperature lower than starting GG and physicochemical properties of obtained hydrogels suggested their potential use in biomedical field.
通过使用双（4-硝基苯基）碳酸酯（4-NPBC）作为偶联剂，通过酰胺键将十八烷基胺（C-NH）共价连接到多糖主链上，制备了结冷胶（GG）的新衍生物。从物理化学性质和新衍生物形成水凝胶的能力方面研究了接枝到高分子量GG亲水性主链上的烷基链的影响。在含有不同种类和浓度离子的溶液中获得了一系列水凝胶，并对其稳定性和机械性能进行了评估。所得到的衍生物在低于起始GG的温度下可溶，所得水凝胶的物理化学性质表明它们在生物医学领域具有潜在用途。
