Leda Ana R, Dygert Levy, Bertrand Luc, Toborek Michal
Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami.
Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami; The Jerzy Kukuczka Academy of Physical Education;
J Vis Exp. 2017 Jan 31(119):54804. doi: 10.3791/54804.
Animal models of central nervous system (CNS) diseases and, consequently, blood-brain barrier disruption diseases, require the delivery of exogenous substances into the brain. These exogenous substances may induce injurious impact or constitute therapeutic strategy. The most common delivery methods of exogenous substances into the brain are based on systemic deliveries, such as subcutaneous or intravenous routes. Although commonly used, these approaches have several limitations, including low delivery efficacy into the brain. In contrast, surgical methods that locally deliver substances into the CNS are more specific and prevent the uptake of the exogenous substances by other organs. Several surgical methods for CNS delivery are available; however, they tend to be very traumatic. Here, we describe a mouse infusion microsurgery technique, which effectively delivers substances into the brain via the internal carotid artery, with minimal trauma and no interference with normal CNS functionality.
中枢神经系统（CNS）疾病的动物模型，以及由此产生的血脑屏障破坏疾病的动物模型，需要将外源性物质输送到大脑中。这些外源性物质可能会产生有害影响或构成治疗策略。将外源性物质输送到大脑中最常见的方法是基于全身给药，如皮下或静脉途径。虽然这些方法常用，但有几个局限性，包括向大脑的递送效率低。相比之下，将物质局部递送至中枢神经系统的手术方法更具特异性，并可防止其他器官摄取外源性物质。有几种用于中枢神经系统递送的手术方法；然而，它们往往具有很大的创伤性。在这里，我们描述了一种小鼠灌注显微手术技术，该技术可通过颈内动脉有效地将物质输送到大脑中，创伤最小且不干扰中枢神经系统的正常功能。
