Hamamoto Kouko, Mizuno Yasuharu
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1, Tokura, Kokubunji, Tokyo 185-8511, Japan.
J Vet Med Sci. 2017 Mar 18;79(3):474-478. doi: 10.1292/jvms.15-0350. Epub 2017 Feb 10.
We assessed ampicillin (ABPC) concentrations of liver, kidney and skin at a 2-day withdrawal period in ten male and ten female White Leghorn chickens fed the diet containing ABPC (ABPC medicated feed 40 mg/kg body weight/day) for a week. The ABPC residues were measured with liquid chromatography-tandem mass spectrometry and the mean recoveries and quantitation limits ranged from 93.0% to 102.7% and from 0.1 to 1.4 ng/g, respectively. The residual ABPC concentrations were ≤7.82 ng/g for the skin and ≤0.64 ng/g for the kidney, suggesting below the Japanese provisional maximum residue limits. These results revealed that the analytical method is developed for residue ABPC and that the withdrawal period is appropriate.
我们对10只雄性和10只雌性白来航鸡进行了评估，这些鸡连续一周喂食含氨苄青霉素（ABPC）的饲料（ABPC药饲，40毫克/千克体重/天），在停药2天时检测其肝脏、肾脏和皮肤中的ABPC浓度。采用液相色谱-串联质谱法测定ABPC残留量，平均回收率和定量限分别为93.0%至102.7%和0.1至1.4纳克/克。皮肤中ABPC残留浓度≤7.82纳克/克，肾脏中≤0.64纳克/克，表明低于日本暂定最大残留限量。这些结果表明已建立了ABPC残留分析方法，且停药期合适。
