Zhu Wenping, Dai Liyan, Liu Zengchen, Yang Weijie, Zhao Chunxiang, Li Yanxia, Chen Yahong
College of Chemistry and Chemical Engineering, Zhoukou Normal University.
Anal Sci. 2017;33(2):203-207. doi: 10.2116/analsci.33.203.
DNA-templated copper nanoparticles (CuNPs) have recently received considerable interest as functional fluorescent probes for biochemical analysis. In this work, a novel ATP-dependent ligation reactions (ATP-DLR) based ATP assay strategy was proposed by using hairpin-shaped (HS) DNA templated CuNPs as a fluorescent probe. Nick sealing by T4 DNA ligase leads to the formation of intact HS DNA, which can resist the exonuclease cleavage and be taken as the template for CuNPs formation, resulting in strong fluorescence. The proposed ATP detection is label free, sensitive and highly selective, and it has good linearity from 0.02 to 4 μM and a detection limit of 7 nM. This strategy is expected to promote the exploitation and application of DNA-templated CuNPs in biochemical and biomedical studies, and holds great promise in fluorescence detection for other ligation-related biomolecules.
基于DNA模板的铜纳米颗粒（CuNPs）作为用于生化分析的功能性荧光探针，最近受到了广泛关注。在这项工作中，提出了一种基于新型ATP依赖性连接反应（ATP-DLR）的ATP检测策略，该策略使用发夹形（HS）DNA模板化的CuNPs作为荧光探针。T4 DNA连接酶进行的缺口封闭导致完整HS DNA的形成，其可以抵抗核酸外切酶的切割，并作为CuNPs形成的模板，从而产生强荧光。所提出的ATP检测无需标记、灵敏且具有高度选择性，在0.02至4 μM范围内具有良好的线性，检测限为7 nM。该策略有望促进DNA模板化CuNPs在生化和生物医学研究中的开发和应用，并在其他与连接相关的生物分子的荧光检测中具有广阔前景。
