Xu Gang, Zhu Yuhua, Pang Jie
Pharmaceutical Research Center and School of Chemistry and Chemical Engineering, Southeast University.
Anal Sci. 2017;33(2):223-227. doi: 10.2116/analsci.33.223.
A simple and sensitive method to determinate glucose content was developed based on single plasmonic nanoparticles by conventional dark-field microscopy (DFM). An enzyme-responsive plasmonic Ag/Au bilayer of rods was designed and prepared. Their localized surface plasmon resonance (LSPR) could be tailored by the enzyme reaction of glucose oxidase (GOx) by finely tuning the morphology and plasmonic optical response of the hybrid nanostructure. It was found that the plasmon resonance scattering (PRS) spectra peak (λ) shifted to longer wavelengths under enzyme reactions, and the degree of the shift were proportional to the content of glucose. This approach is convenient to study the local concentration of glucose in real time.
基于传统暗场显微镜（DFM），开发了一种基于单等离子体纳米颗粒测定葡萄糖含量的简单灵敏方法。设计并制备了一种酶响应性的棒状等离子体Ag/Au双层结构。通过精细调节混合纳米结构的形态和等离子体光学响应，其局域表面等离子体共振（LSPR）可由葡萄糖氧化酶（GOx）的酶反应来调控。研究发现，在酶反应下，等离子体共振散射（PRS）光谱峰（λ）向更长波长移动，且移动程度与葡萄糖含量成正比。该方法便于实时研究葡萄糖的局部浓度。
