Jakobsen Sashia L, Williams Catherine J A, Wang Tobias, Bertelsen Mads F
Zoophysiology, Institute for Bioscience, Aarhus University, C.F. Møllers Allé 3, 8000 Aarhus C, Denmark.
Zoophysiology, Institute for Bioscience, Aarhus University, C.F. Møllers Allé 3, 8000 Aarhus C, Denmark.
Comp Biochem Physiol A Mol Integr Physiol. 2017 May;207:30-35. doi: 10.1016/j.cbpa.2017.02.012. Epub 2017 Feb 10.
Mechanical ventilation is widely recommended for reptiles during anesthesia, and while it is well-known that their low ectothermic metabolism requires much lower ventilation than in mammals, very little is known about the influence of ventilation protocol on the recovery from anesthesia. Here, 15 ball pythons (Python regius) were induced and maintained with isoflurane for 60min at one of three ventilation protocols (30, 125, or 250mlminkg body mass) while an arterial catheter was inserted, and ventilation was then continued on 100% oxygen at the specified rate until voluntary extubation. Mean arterial blood pressure and heart rate (HR) were measured, and arterial blood samples collected at 60, 80, 180min and 12 and 24h after intubation. In all three groups, there was evidence of a metabolic acidosis, and snakes maintained at 30mlminkg experienced an additional respiratory acidosis, while the two other ventilation protocols resulted in normal or low arterial PCO. In general, normal acid-base status was restored within 12h in all three protocols. HR increased by 143±64% during anesthesia with high mechanical ventilation (250mlminkg) in comparison with recovered values. Recovery times after mechanical ventilation at 30, 125, or 250mlminkg were 289±70, 126±16, and 68±7min, respectively. Mild overventilation may result in a faster recovery, and the associated lowering of arterial PCO normalised arterial pH in the face of metabolic acidosis.
在麻醉期间，机械通气被广泛推荐用于爬行动物。虽然众所周知，它们的低体温代谢所需的通气量比哺乳动物低得多，但关于通气方案对麻醉恢复的影响却知之甚少。在此，15条球蟒（球蟒）在三种通气方案（30、125或250毫升/分钟/千克体重）之一的条件下，用异氟醚诱导并维持麻醉60分钟，同时插入动脉导管，然后以指定速率持续给予100%氧气通气，直至自主拔管。测量平均动脉血压和心率（HR），并在插管后60、80、180分钟以及12和24小时采集动脉血样。在所有三组中，均有代谢性酸中毒的迹象，维持在30毫升/分钟/千克的蛇还出现了额外的呼吸性酸中毒，而其他两种通气方案导致动脉PCO正常或降低。一般来说，所有三种方案在12小时内酸碱状态恢复正常。与恢复值相比，在高机械通气（250毫升/分钟/千克）麻醉期间，HR增加了143±64%。在30、125或250毫升/分钟/千克的机械通气后，恢复时间分别为289±70、126±16和68±7分钟。轻度过度通气可能导致更快的恢复，并且在代谢性酸中毒的情况下，动脉PCO的降低使动脉pH值正常化。
