Department of Physics, King's College London, Strand, London, WC2R 2LS, UK.
Methods Appl Fluoresc. 2016 Nov 11;4(4):045001. doi: 10.1088/2050-6120/4/4/045001.
The transition dipole moment of organic dyes PM546 and rhodamine 123 is calculated from fluorescence lifetime measurements in solutions of different refractive index. A model proposed by Toptygin et al (2002 J. Phys. Chem. B 106 3724-34) provides a relationship between the radiative rate constant and refractive index of the solvent, and allows the electronic transition dipole moments to be found: it is (7.1 ± 1.1) D for PM546 which matches that found in the literature, and (8.1 ± 0.1) D for rhodamine 123. Toptygin's model goes further in predicting the shape of the fluorescent dye and here we predict the shape of PM546 and rhodamine 123 to be ellipsoidal.
从不同折射率溶液中的荧光寿命测量中计算出有机染料 PM546 和罗丹明 123 的跃迁偶极矩。Toptygin 等人（2002 年 J. Phys. Chem. B 106 3724-34）提出的模型提供了辐射速率常数与溶剂折射率之间的关系，并允许找到电子跃迁偶极矩：PM546 的为（7.1 ± 1.1）D，与文献中发现的一致，而罗丹明 123 的为（8.1 ± 0.1）D。Toptygin 的模型更进一步预测了荧光染料的形状，在这里我们预测 PM546 和罗丹明 123 的形状为椭圆形。
