Institute of Molecular Medicine, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX 76107, USA. Department of Food Science, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
Methods Appl Fluoresc. 2016 Nov 17;4(4):047001. doi: 10.1088/2050-6120/4/4/047001.
A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.
使用带有长寿命的荧光寿命成像探针，并结合时间门控技术，以透明质酸作为探针模板来检测透明质酸酶。该探针是通过用长寿命的吖啶二氧杂环戊烯荧光团（ADOTA）对透明质酸进行重标记而开发的。我们使用该探针来对前列腺癌细胞 DU-145 产生的透明质酸酶进行成像。
