De Fabritiis P, Ferrero D, Sandrelli A, Tarella C, Meloni G, Pulsoni A, Pregno P, Badoni R, De Felice L, Gallo E
Department of Human Biopathology, University La Sapienza, Rome, Italy.
Bone Marrow Transplant. 1989 Nov;4(6):669-74.
A mouse monoclonal antibody (S4-7) reacting with human myelomonocytic cells has been previously shown to be suitable for bone marrow purging in selected acute myelogenous leukemia (AML) patients with S4-7 positive leukemic clonogenic cells at diagnosis. The results obtained in seven AML patients who underwent such a treatment, followed by autologous bone marrow transplantation (ABMT), are now reported. Six patients underwent ABMT in first complete remission (CR), one in second CR, after BAVC conditioning regimen. One patient died of infection 1 month after ABMT; in the other six a complete recovery of hemopoiesis was observed. In spite of S4-7 reactivity with normal myelomonocytic cells, a prompt recovery of granulopoiesis was however observed both in in vitro liquid culture and in vivo with a median time of 20 days to reach granulocyte values of 500 x 10(6)/l. The patient transplanted in 2nd CR relapsed 3 months after ABMT. Of the five evaluable patients transplanted in 1st CR, two relapsed 8 and 9 months post-ABMT while three remain in continuous CR at 35, 47, 57 months. Leukemic cells of two of the three patients with recurrent disease were studied at relapse and in both could be detected a significant percentage of S4-7 negative cells, detectable neither at diagnosis nor (one patient) at the time of first relapse after standard chemotherapy.
一种与人类骨髓单核细胞反应的小鼠单克隆抗体（S4-7）先前已被证明适用于某些急性髓性白血病（AML）患者的骨髓净化，这些患者在诊断时存在S4-7阳性白血病克隆形成细胞。现将7例接受这种治疗并随后进行自体骨髓移植（ABMT）的AML患者的结果报告如下。6例患者在首次完全缓解（CR）时接受ABMT，1例在第二次CR时接受，采用BAVC预处理方案。1例患者在ABMT后1个月死于感染；其他6例患者造血功能完全恢复。尽管S4-7与正常骨髓单核细胞有反应，但在体外液体培养和体内均观察到粒细胞生成迅速恢复，达到粒细胞值500×10⁶/L的中位时间为20天。在第二次CR时接受移植的患者在ABMT后3个月复发。在首次CR时接受移植的5例可评估患者中，2例在ABMT后8个月和9个月复发，而3例在35、47、57个月时仍处于持续CR状态。对3例复发患者中的2例复发时的白血病细胞进行了研究，在两者中均检测到相当比例的S4-7阴性细胞，这些细胞在诊断时以及（1例患者）标准化疗后的首次复发时均未检测到。
