Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil.
FI-TRACE Group, Department of Chemistry, Faculty of Sciences, University of the Balearic Islands, Carretera de Valldemossa km.7.5, E-07122 Palma de Mallorca, Spain.
Food Chem. 2017 Jun 15;225:125-131. doi: 10.1016/j.foodchem.2017.01.017. Epub 2017 Jan 5.
In this paper, Cu, Fe, Mn and Zn contents in transgenic (T - MSOY7122RR) and non-transgenic (NT - MSOY8200) soybean seeds, sown at summer and winter cultivation periods are investigated using four microwave decomposition methods. Student's t tests demonstrate significant differences (p=0.05; n=4), for Cu, Mn and Zn (namely, 8, 9 and 26% higher concentrations in T compared to NT seeds, respectively). Through principal component analysis, precursor and successor soybean seeds are identified. Cu is demonstrated to play an important role in the differentiation of the cultivars, whereas Fe and Zn are of particular relevance in the classification of seeds cultivated in winter against those in summer. Using in vitro extraction based on the Unified Bioaccessibility Method, the bioaccessibility of the above nutrients is proven to differ in both the gastric and gastrointestinal phases on the basis of the transgenesis and the cultivation periods.
本文采用四种微波消解方法研究了夏季和冬季种植的转基因（T-MSOY7122RR）和非转基因（NT-MSOY8200）大豆种子中的铜、铁、锰和锌含量。学生 t 检验表明，铜、锰和锌在转基因种子中的浓度分别比非转基因种子高 8%、9%和 26%（p=0.05；n=4）。通过主成分分析，可以区分前体和后继大豆种子。结果表明，铜在品种分化中起着重要作用，而铁和锌在冬季和夏季种植的种子分类中具有特别重要的意义。通过基于统一生物可利用性方法的体外提取，证明了基于转基因和种植期，在胃和胃肠道阶段，上述营养素的生物可利用性存在差异。
