Kulwas Arleta, Lisewska Beata, Jundziłł Wiesław, Ruszkowska Barbara, Drewniak Wanda, Ruprecht Zofia, Gadomska Grażyna, Rość Danuta
Department of Pathophysiology, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, Poland.
Department of Pathophysiology, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, Poland.
Adv Med Sci. 2017 Mar;62(1):87-91. doi: 10.1016/j.advms.2016.07.007. Epub 2017 Feb 11.
The aim was to evaluate tissue plasminogen activator (tPA) and plasminogen activator inhibitor type 1 (PAI-1) concentration using enzyme linked immunosorbent assay method (ELISA) in diabetic foot syndrome (DFS) as compared to a group of healthy people and patients with diabetes mellitus without symptomatic vascular complications (DM2T).
MATERIAL/METHODS: Venous blood samples were collected from 90 patients with type 2 diabetes mellitus (30 - DM2T; 60 - DFS). Age-matched controls were also included (n=30). tPA and PAI-1 plasma levels were measured by enzyme-linked immunosorbent assay (ELISA).
We found a significantly lower concentration of tPA:Ag in patients with DFS in comparison to the DM2T group; tPA concentrations were significantly higher in DM2T as compared to the control group. We observed significantly lower concentration of PAI-1:Ag in DF patients treated for hypertension as compared to patients without hypertension. The tPA:Ag and PAI-1:Ag concentration analysis in DFS depending on age, gender and BMI did not show any significant differences.
A lower concentration of tPA in patients with DFS may be associated with damage to the endothelial cells, especially in the microvasculature, and the sympathetic nervous system.
旨在采用酶联免疫吸附测定法（ELISA）评估糖尿病足综合征（DFS）患者组织型纤溶酶原激活剂（tPA）和纤溶酶原激活物抑制剂1型（PAI-1）的浓度，并与一组健康人和无血管并发症症状的糖尿病患者（DM2T）进行比较。
材料/方法：采集90例2型糖尿病患者（30例DM2T；60例DFS）的静脉血样本。还纳入了年龄匹配的对照组（n = 30）。采用酶联免疫吸附测定法（ELISA）检测tPA和PAI-1的血浆水平。
我们发现，与DM2T组相比，DFS患者的tPA:Ag浓度显著降低；与对照组相比，DM2T组的tPA浓度显著升高。我们观察到，与未患高血压的患者相比，接受高血压治疗的DFS患者的PAI-1:Ag浓度显著降低。根据年龄、性别和体重指数对DFS患者的tPA:Ag和PAI-1:Ag浓度进行分析，未发现任何显著差异。
DFS患者tPA浓度较低可能与内皮细胞损伤有关，尤其是在微血管系统和交感神经系统中。
