嗜麦芽窄食单胞菌中SmeDEF外排泵的过表达降低氨基糖苷类耐药性
Overexpression of SmeDEF Efflux Pump Decreases Aminoglycoside Resistance in Stenotrophomonas maltophilia.
作者信息
Huang Yi-Wei, Lin Cheng-Wen, Ning Hsiao-Chen, Lin Yi-Tsung, Chang Yi-Chih, Yang Tsuey-Ching
机构信息
Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan.
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
出版信息
Antimicrob Agents Chemother. 2017 Apr 24;61(5). doi: 10.1128/AAC.02685-16. Print 2017 May.
Abstract
The SmeDEF pump of is negatively regulated by SmeT. In this study, strains KJΔT ( deletion mutant) and KJT-D (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-D, downregulated expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.
摘要
的SmeDEF泵受到SmeT的负调控。在本研究中，菌株KJΔT（缺失突变体）和KJT-D（具有缺陷的SmeT结合位点的突变体）对氯霉素/萘啶酸/大环内酯类药物的抗性增加，而对氨基糖苷类药物敏感。在KJΔT或KJT-D中过表达SmeDEF泵会下调负责降低氨基糖苷类抗性的表达。此外，响应于SmeDEF过表达，SmeRySy双组分调节系统被下调，这支持了它参与调节回路。
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