Zarbin M A, Baraban J, Worley P
Wilmer Ophthalmological Institute, Johns Hopkins Hospital, Baltimore, MD 21205.
Brain Res. 1989 Sep 18;497(2):334-43. doi: 10.1016/0006-8993(89)90279-5.
We have localized the distribution of [3H]forskolin and [3H]phorbol dibutyrate binding sites autoradiographically in the rat, monkey, and human retina. In the rat and monkey retina, forskolin binding was enriched in the inner plexiform layer, in the inner and outer segments of the photoreceptors, and in the retinal pigment epithelium. In the human retina, forskolin binding sites were uniformly distributed and higher in density. Forskolin binding was also detected over the ciliary body, the ciliary epithelium, and the iris sphincter. The distribution of phorbol ester binding sites was similar in the rat, monkey, and human retina. The inner plexiform layer contained the highest density followed by the inner nuclear and outer plexiform layers, and the ganglion cell layer. In the rat, phorbol ester binding was present in the iris, the ciliary body, and the ciliary epithelium. The monkey and human ciliary body also contained a low density of phorbol ester binding sites.
我们已通过放射自显影法在大鼠、猴子和人类视网膜中定位了[3H]福斯高林和[3H]佛波醇二丁酸酯结合位点的分布。在大鼠和猴子视网膜中，福斯高林结合在神经节细胞层、内核层和外网状层，光感受器的内、外段以及视网膜色素上皮中富集。在人类视网膜中，福斯高林结合位点分布均匀且密度更高。在睫状体、睫状上皮和虹膜括约肌上也检测到了福斯高林结合。大鼠、猴子和人类视网膜中佛波醇酯结合位点的分布相似。内网状层密度最高，其次是内核层和外网状层以及神经节细胞层。在大鼠中，虹膜、睫状体和睫状上皮中存在佛波醇酯结合。猴子和人类的睫状体中也含有低密度的佛波醇酯结合位点。
