State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University , Changsha, Hunan 410082, P. R. China.
Institute of Pharmacy and Pharmacology, University of South China , Hengyang, Hunan 421001, P. R. China.
Anal Chem. 2017 Mar 7;89(5):2692-2697. doi: 10.1021/acs.analchem.6b03844. Epub 2017 Feb 24.
Understanding the heterogeneous distribution of the physical and chemical properties of plasmonic metal nanoparticles is fundamentally important to their basic and applied research. Traditionally, they are obtained either indirectly via bulk spectroscopic measurements plus electron microscopic characterizations or through single molecule/particle imaging of nanoparticles immobilized on planar substrates. In this study, by using light-sheet scattering microscopy with a supercontinuum white laser, highly sensitive imaging of individual metal nanoparticles (MNPs) flowing inside a capillary, driven by either pressure or electric field, was achieved for the first time. We demonstrate that single plasmonic nanoparticles with different size or chemical modification could be differentiated through their electrophoretic mobility in a few minutes. This technique could potentially be applied to high throughput characterization and evaluation of single metal nanoparticles as well as their dynamic interactions with various local environments.
了解等离子体金属纳米粒子的物理和化学性质的不均匀分布对于它们的基础研究和应用研究至关重要。传统上，它们要么通过体光谱测量加上电子显微镜表征间接获得，要么通过固定在平面衬底上的纳米粒子的单分子/粒子成像获得。在这项研究中，我们首次使用超连续白光激光的光片散射显微镜，实现了在压力或电场驱动下，在毛细管内流动的单个金属纳米粒子（MNPs）的高灵敏度成像。我们证明，通过它们在几分钟内的电泳迁移率，可以区分具有不同尺寸或化学修饰的单个等离子体纳米粒子。该技术有可能应用于高通量表征和评估单个金属纳米粒子及其与各种局部环境的动态相互作用。
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