Chai Lihong, Wang Hongyuan, Zhao Hongfeng, Dong Suiming
School of Environmental Science and Engineering, Chang'an University, Xi'an, 710054, People's Republic of China.
Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region, Ministry of Education, Chang'an University, Xi'an, 710054, China.
Bull Environ Contam Toxicol. 2017 Apr;98(4):496-501. doi: 10.1007/s00128-017-2036-3. Epub 2017 Feb 14.
Bufo gargarizans tadpoles were chronically exposed to waterborne fluoride at measured concentrations ranging from 0.4 to 61.2 mg F/L for 70 days from Gosner stage 26 to completion of metamorphosis. The chronic exposure caused a concentration-dependent mortality in all tested fluoride concentrations. Total length, snout-to-vent length (SVL), body mass, and developmental stage of tadpoles were significantly inhibited at 42.6 mg F/L. In addition, significant metamorphic delay and increase in size at completion of metamorphosis occurred after exposure to 19.8 mg F/L. Moreover, 19.8 mg F/L suppressed the bone mineralization of larvae at completion of metamorphosis. However, the bone mineralization could be enhanced by 4.1 mg F/L. In conclusion, our results suggested that the presence of high concentrations of fluoride could increase mortality risk, delay metamorphosis, and suppress skeletal ossification in B. gargarizans larvae.
从戈斯纳26期开始，将中华大蟾蜍蝌蚪长期暴露于浓度为0.4至61.2毫克氟/升的水体氟化物中，持续70天，直至变态完成。在所有测试的氟化物浓度下，慢性暴露均导致浓度依赖性死亡。在42.6毫克氟/升时，蝌蚪的全长、吻肛长度（SVL）、体重和发育阶段均受到显著抑制。此外，暴露于19.8毫克氟/升后，出现了显著的变态延迟和变态完成时体型增大的情况。而且，19.8毫克氟/升抑制了变态完成时幼虫的骨矿化。然而，4.1毫克氟/升可增强骨矿化。总之，我们的结果表明，高浓度氟化物的存在会增加中华大蟾蜍幼虫的死亡风险、延迟变态并抑制骨骼骨化。
