Richard Patricia, Vethantham Vasupradha, Manley James L
Department of Biological Sciences, Columbia University, New York, NY, 10027, USA.
Adv Exp Med Biol. 2017;963:15-33. doi: 10.1007/978-3-319-50044-7_2.
SUMO has gained prominence as a regulator in a number of cellular processes. The roles of sumoylation in RNA metabolism, however, while considerable, remain less well understood. In this chapter we have assembled data from proteomic analyses, localization studies and key functional studies to extend SUMO's role to the area of mRNA processing and metabolism. Proteomic analyses have identified multiple putative sumoylation targets in complexes functioning in almost all aspects of mRNA metabolism, including capping, splicing and polyadenylation of mRNA precursors. Possible regulatory roles for SUMO have emerged in pre-mRNA 3' processing, where SUMO influences the functions of polyadenylation factors and activity of the entire complex. SUMO is also involved in regulating RNA editing and RNA binding by hnRNP proteins, and recent reports have suggested the involvement of the SUMO pathway in mRNA export. Together, these reports suggest that SUMO is involved in regulation of many aspects of mRNA metabolism and hold the promise for exciting future studies.
SUMO作为多种细胞过程的调节因子已备受关注。然而，尽管SUMO化在RNA代谢中的作用显著，但其仍未得到充分了解。在本章中，我们汇集了蛋白质组学分析、定位研究和关键功能研究的数据，以将SUMO的作用扩展到mRNA加工和代谢领域。蛋白质组学分析已在几乎参与mRNA代谢各个方面的复合物中鉴定出多个可能的SUMO化靶点，包括mRNA前体的加帽、剪接和聚腺苷酸化。SUMO在mRNA前体3'加工中可能具有调节作用，其中SUMO会影响聚腺苷酸化因子的功能以及整个复合物的活性。SUMO还参与调节RNA编辑和hnRNP蛋白的RNA结合，最近的报道表明SUMO途径参与mRNA输出。这些报道共同表明，SUMO参与了mRNA代谢多个方面的调节，并为未来令人兴奋的研究带来了希望。
