College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University , 700 Changcheng Road, Qingdao 266109, China.
Institute for Biosensing & In-Vitro Diagnostics and College of Chemistry & Chemical Engineering, Qingdao University , 308 Ningxia Road, Qingdao 266071, China.
ACS Appl Mater Interfaces. 2017 Mar 1;9(8):6894-6901. doi: 10.1021/acsami.6b15992. Epub 2017 Feb 15.
To improve the biosensing performance of organophosphorus hydrolase (OPH), the novel bioinorganic hybrid catalysts were facilely explored by biomineralization and cell surface display technology. During biomineralization, cobalt phosphate crystals were deposited onto the surface of OPH-fused bacteria, and the inorganic crystals at middle of cell collapsed inwardly to form the final spindle morphology because of the lowest energy principle and the mechanics principle. OPH would show the allosteric effect from "inactive" form to "active" form, and the "active" form was "fixed" when OPH was embedded into cobalt phosphate. Therefore, the activity of mineralized OPH-fused cells was greatly enhanced about 3 times in comparison with original OPH-fused cells. Additionally, the stability of the novel hybrid catalysts was also significantly improved. Further, the as-synthesized bioinorganic hybrid catalysts were applied to sensitive paraoxon biosensing, which exhibited lower limit of detection than that of the original counterpart. Thus, this hybrid biocatalytic system would provide a model to develop a wide range of biocatalysts and find a wide range of applications in industrial catalysis, analytical chemistry, and environmental engineering.
为了提高有机磷水解酶（OPH）的生物传感性能，本研究通过生物矿化和细胞表面展示技术，简便地探索了新型生物无机杂化催化剂。在生物矿化过程中，磷酸钴晶体沉积在融合了 OPH 的细菌表面，由于最低能量原理和力学原理，细胞中间的无机晶体向内坍塌，形成最终的纺锤形形态。OPH 会表现出从“无活性”形式到“活性”形式的变构效应，而当 OPH 嵌入磷酸钴时，“活性”形式被“固定”。因此，与原始融合 OPH 的细胞相比，矿化融合 OPH 的细胞的活性大大提高了约 3 倍。此外，新型杂化催化剂的稳定性也得到了显著提高。此外，所合成的生物无机杂化催化剂被应用于灵敏的对氧磷生物传感，其检测限低于原始对应物。因此，该杂化生物催化体系将为开发广泛的生物催化剂提供模型，并在工业催化、分析化学和环境工程中找到广泛的应用。
