He L H, Xiao E, An J G, He Y, Chen S, Zhao L, Zhang T, Zhang Y
1 Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, China.
2 National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, China.
J Dent Res. 2017 May;96(5):539-546. doi: 10.1177/0022034517691507. Epub 2017 Feb 15.
Treatment of bisphosphonate-related osteonecrosis of the jaw (BRONJ) has posed significant challenges to maxillofacial surgeons because of the poor repair of BRONJ bone defects. Moreover, the pathological mechanisms remain unclear. Bone marrow stromal cells (BMSCs) play key roles during bone repair and bone regeneration. However, the activities of BMSCs derived from BRONJ lesions and the BRONJ lesion boundary, as well as the roles of BMSCs in BRONJ defect repair, are poorly defined. In this study, we found that BMSCs from the central area of the osteonecrotic BRONJ region (center-BRONJ BMSCs) and the peripheral area at the recommended debridement boundary (peri-BRONJ BMSCs) had decreased proliferative ability, self-renewal capacity, and multidifferentiation capacities compared with control BMSCs. Osteoclast-inducing ability was also impaired in BRONJ BMSCs. All of these results suggested that the decreased activities of BRONJ BMSCs, even the BMSCs derived from the BRONJ lesion boundary, might be an important factor leading to insufficient bone repair of BRONJ lesions. This study offers early stage evidence for the use of marrow stromal cells in the treatment of BRONJ.
双膦酸盐相关颌骨骨坏死（BRONJ）的治疗给颌面外科医生带来了重大挑战，因为BRONJ骨缺损修复不佳。此外，其病理机制仍不清楚。骨髓间充质干细胞（BMSCs）在骨修复和骨再生过程中起关键作用。然而，源自BRONJ病变及BRONJ病变边界的BMSCs的活性，以及BMSCs在BRONJ缺损修复中的作用，目前尚不清楚。在本研究中，我们发现与对照BMSCs相比，来自骨坏死性BRONJ区域中心（中心-BRONJ BMSCs）和推荐清创边界外周区域（外周-BRONJ BMSCs）的BMSCs的增殖能力、自我更新能力和多向分化能力均下降。BRONJ BMSCs的破骨细胞诱导能力也受损。所有这些结果表明，BRONJ BMSCs活性下降，即使是源自BRONJ病变边界的BMSCs，可能是导致BRONJ病变骨修复不足的重要因素。本研究为使用骨髓间充质干细胞治疗BRONJ提供了早期证据。
