一种用于定量测定人血浆中两性霉素B的快速且可靠的超高效液相色谱-二极管阵列检测法。

A rapid and robust UHPLC-DAD method for the quantification of amphotericin B in human plasma.

作者信息

Barco Sebastiano, Zunino Alessia, D'Avolio Antonio, Barbagallo Laura, Maffia Angelo, Tripodi Gino, Castagnola Elio, Cangemi Giuliana

机构信息

Clinical Pathology Laboratory Unit, Istituto Giannina Gaslini, Genoa, Italy.

Department of Medical Sciences, University of Turin, Laboratory of Clinical Pharmacology and Pharmacogenetics, Amedeo di Savoia Hospital, Turin, Italy.

出版信息

J Pharm Biomed Anal. 2017 May 10;138:142-145. doi: 10.1016/j.jpba.2017.01.048. Epub 2017 Feb 8.

DOI:10.1016/j.jpba.2017.01.048

PMID:28199895

Abstract

Amphotericin B is an antifungal drug widely used in Intensive Care Units. Therapeutic drug monitoring (TDM) of amphotericin B is recommended for the assessment of toxicity surveillance and treatment optimization. In this paper we described the development and validation of a new Ultra High Performance Liquid Chromatography coupled to Diode Array Detection (UHPLC-DAD) method for the quantification of Amphotericin B in 200μL human plasma over a wide range of concentrations (0.125-10mg/L). The new method has been validated following international guidelines on bioanalytical method validation and showed high selectivity, high accuracy and precision and high process efficiency. The new UHPLC-DAD method that we describe is robust, rapid, cost effective and suitable for application to the routine TDM analyses.

摘要

两性霉素B是一种在重症监护病房广泛使用的抗真菌药物。建议对两性霉素B进行治疗药物监测（TDM），以评估毒性监测和优化治疗。在本文中，我们描述了一种新的超高效液相色谱-二极管阵列检测（UHPLC-DAD）方法的开发和验证，该方法用于在宽浓度范围（0.125-10mg/L）内定量测定200μL人血浆中的两性霉素B。该新方法已按照生物分析方法验证的国际指南进行了验证，具有高选择性、高准确度和精密度以及高处理效率。我们所描述的新UHPLC-DAD方法稳健、快速、具有成本效益，适用于常规TDM分析。