Reimschuessel Renate, Grabenstein Michael, Guag Jake, Nemser Sarah M, Song Kyunghee, Qiu Junshan, Clothier Kristin A, Byrne Barbara A, Marks Stanley L, Cadmus Kyran, Pabilonia Kristy, Sanchez Susan, Rajeev Sreekumari, Ensley Steve, Frana Timothy S, Jergens Albert E, Chappell Kimberly H, Thakur Siddhartha, Byrum Beverly, Cui Jing, Zhang Yan, Erdman Matthew M, Rankin Shelley C, Daly Russell, Das Seema, Ruesch Laura, Lawhon Sara D, Zhang Shuping, Baszler Timothy, Diaz-Campos Dubraska, Hartmann Faye, Okwumabua Ogi
U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland, USA
U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland, USA.
J Clin Microbiol. 2017 May;55(5):1350-1368. doi: 10.1128/JCM.02137-16. Epub 2017 Feb 15.
Eleven laboratories collaborated to determine the periodic prevalence of in a population of dogs and cats in the United States visiting veterinary clinics. Fecal samples (2,965) solicited from 11 geographically dispersed veterinary testing laboratories were collected in 36 states between January 2012 and April 2014 and tested using a harmonized method. The overall study prevalence of in cats (3 of 542) was <1%. The prevalence in dogs (60 of 2,422) was 2.5%. Diarrhea was present in only 55% of positive dogs; however, 3.8% of the all diarrheic dogs were positive, compared with 1.8% of the nondiarrheic dogs. -positive dogs were significantly more likely to have consumed raw food ( = 0.01), to have consumed probiotics ( = 0.002), or to have been given antibiotics ( = 0.01). Rural dogs were also more likely to be positive than urban ( = 0.002) or suburban ( = 0.001) dogs. In the 67 isolates, 27 unique serovars were identified, with three dogs having two serovars present. Antimicrobial susceptibility testing of 66 isolates revealed that only four of the isolates were resistant to one or more antibiotics. Additional characterization of the 66 isolates was done using pulsed-field gel electrophoresis and whole-genome sequencing (WGS). Sequence data compared well to resistance phenotypic data and were submitted to the National Center for Biotechnology Information (NCBI). This study suggests an overall decline in prevalence of -positive dogs and cats over the last decades and identifies consumption of raw food as a major risk factor for infection. Of note is that almost half of the -positive animals were clinically nondiarrheic.
11个实验室合作，以确定美国前往兽医诊所就诊的犬猫群体中[病原体名称]的周期性流行情况。2012年1月至2014年4月期间，从11个地理位置分散的兽医检测实验室征集了2965份粪便样本，这些样本来自36个州，并采用统一方法进行检测。猫的总体研究流行率（542只中的3只）<1%。犬的流行率（2422只中的60只）为2.5%。仅有55%的阳性犬出现腹泻；然而，所有腹泻犬中3.8%为阳性，而非腹泻犬中这一比例为1.8%。[病原体名称]阳性犬食用生食（P = 0.01）、食用益生菌（P = 0.002）或接受过抗生素治疗（P = 0.01）的可能性显著更高。农村犬感染[病原体名称]阳性的可能性也高于城市犬（P = 0.002）或郊区犬（P = 0.001）。在67株分离株中，鉴定出27种独特血清型，有3只犬存在两种血清型。对66株分离株进行的药敏试验表明，只有4株分离株对一种或多种抗生素耐药。使用脉冲场凝胶电泳和全基因组测序（WGS）对66株分离株进行了进一步鉴定。序列数据与耐药表型数据比对良好，并提交至美国国立生物技术信息中心（NCBI）。这项研究表明，在过去几十年中，犬猫[病原体名称]阳性的流行率总体呈下降趋势，并确定食用生食是[病原体名称]感染的主要危险因素。值得注意的是，几乎一半的[病原体名称]阳性动物临床上无腹泻症状。
