Department of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Foodborne Pathog Dis. 2021 Apr;18(4):283-289. doi: 10.1089/fpd.2020.2869. Epub 2021 Feb 9.
Within Ethiopia, there is a lack of information on the genetic relatedness of from cattle, beef, and diarrheic patients and its potential transmission from cattle to humans through consumption of contaminated beef. The objective of this study was to assess the prevalence and determine the serotypes, genetic relatedness, and antimicrobial resistance of in cattle in two local slaughterhouses, in beef at retail shops, and in diarrheic patients in the only hospital in Bishoftu, Ethiopia. was detected in 2.5% (6/240) of cattle samples, in 8.7% (11/127) of beef samples, and in 2.3% (5/216) of the diarrheic patients. Four serotypes: Typhimurium, Eastbourne, Saintpaul, and Cotham were identified. Typhimurium and Eastbourne were isolated from cattle and beef, whereas Saintpaul and Cotham were isolated only from diarrheic patients. Except for serotype Saintpaul, all isolates were grouped into five pulsotypes, of which two pulsotypes contained isolates from cattle and beef. Isolates from humans represented unique pulsotypes. Among the 22 isolates tested, 95.5% were resistant to at least 1 of the 14 antimicrobials tested. Three isolates originating from cattle were multidrug resistant. One human isolate was susceptible to all antimicrobials tested. More specifically, resistance to ampicillin, sulfamethoxazole, tetracycline, tigecycline, and trimethoprim were observed. The most frequently observed resistance was to sulfamethoxazole (90.9%, 20/22) followed by trimethoprim (22.7%, 5/22). The study revealed considerable contamination of beef at retail shops, antimicrobial resistance to commonly used antimicrobials, and shared genetically similar serotypes between cattle and beef; the link with humans could not be established. Still, the findings of in cattle and beef, the propensity of transfer of from cattle to beef coupled with the common consumption of raw/undercooked beef are likely to pose public health risk in Ethiopia.
在埃塞俄比亚，缺乏有关来自牛、牛肉和腹泻患者的 的遗传相关性的信息，也缺乏其通过食用受污染的牛肉从牛传播给人类的潜在信息。本研究的目的是评估在埃塞俄比亚比绍夫图的唯一一家医院的腹泻患者、两家当地屠宰场的牛以及零售商店的牛肉中 的流行率，并确定其血清型、遗传相关性和抗药性。在 240 份牛样本中检测到 2.5%（6/240）、在 127 份牛肉样本中检测到 8.7%（11/127）、在 216 份腹泻患者样本中检测到 2.3%（5/216）。鉴定出 4 种 血清型：鼠伤寒血清型、伊斯特本血清型、圣保罗血清型和科瑟姆血清型。鼠伤寒血清型和伊斯特本血清型从牛和牛肉中分离出来，而圣保罗血清型和科瑟姆血清型仅从腹泻患者中分离出来。除圣保罗血清型外，所有分离株都被分为 5 个脉冲型，其中 2 个脉冲型包含来自牛和牛肉的分离株。来自人类的分离株代表了独特的脉冲型。在 22 株测试的分离株中，95.5%对测试的 14 种抗生素中的至少 1 种有耐药性。3 株来自牛的分离株为多药耐药株。1 株来自人类的分离株对所有测试的抗生素均敏感。具体而言，观察到对氨苄西林、磺胺甲恶唑、四环素、替加环素和甲氧苄啶的耐药性。最常见的耐药性是磺胺甲恶唑（90.9%，20/22），其次是甲氧苄啶（22.7%，5/22）。研究表明，零售商店的牛肉受到严重污染，对常用抗生素的耐药性，以及牛和牛肉之间遗传上相似的 血清型，与人类之间的联系尚无法确定。尽管如此，在牛和牛肉中发现 ，从牛向牛肉转移的倾向，再加上生/未煮熟牛肉的普遍食用，可能在埃塞俄比亚构成公共卫生风险。
