Foy M R, Stanton M E, Levine S, Thompson R F
Behav Neural Biol. 1987 Jul;48(1):138-49. doi: 10.1016/s0163-1047(87)90664-9.
A number of hormones secreted from the pituitary-adrenal system during stress affect learning and memory processes. The phenomenon of hippocampal long-term potentiation (LTP) is viewed by many as a putative mechanism of memory storage and has proved a most valuable model for study of neuronal plasticity at the cellular level. The present study was conducted to investigate the possibility that stressful events which occur prior (in vivo) to the preparation of brain slices may influence the electrophysiology of the in vitro hippocampal explant when tested for LTP. Adult male rats (Long-Evans male X Sprague-Dawley female) were pair-housed 1 week prior to testing. One animal in each pair was either placed in a restraining tube for 30 min and received no tail shocks (Restraint) or placed in a restraining tube and received tail shocks (1 microA, 1 s) every minute for 30 min (Restraint + Shock). The other animal in each pair was taken directly from the home cage and received no restraint or tail shock (Control). In vitro hippocampal slices were then prepared immediately from these animals according to standard methods. Our results demonstrate a marked impairment of LTP in hippocampal explants taken from rats exposed to stress. The significance of this result with respect to cellular mechanisms underlying the relationship between stress, cognition, and learning is discussed.
应激期间从垂体 - 肾上腺系统分泌的多种激素会影响学习和记忆过程。许多人认为海马体长期增强（LTP）现象是一种假定的记忆存储机制，并且已被证明是在细胞水平研究神经元可塑性的最有价值的模型。本研究旨在调查在制备脑片之前（体内）发生的应激事件在测试LTP时是否可能影响体外海马体植块的电生理学。成年雄性大鼠（长 Evans 雄性×斯普拉 - 道利雌性）在测试前1周成对饲养。每对中的一只动物要么被放入约束管中30分钟且不接受尾部电击（约束组），要么被放入约束管中并每分钟接受尾部电击（1微安，1秒），持续30分钟（约束 + 电击组）。每对中的另一只动物直接从饲养笼中取出，不接受约束或尾部电击（对照组）。然后根据标准方法立即从这些动物制备体外海马体切片。我们的结果表明，从遭受应激的大鼠获取的海马体植块中LTP明显受损。讨论了该结果对于应激、认知和学习之间关系的潜在细胞机制的意义。
