培养的大鼠少突胶质细胞和星形胶质细胞中可溶性神经生长因子受体的结合常数。

Binding constants of soluble NGF-receptors in rat oligodendrocytes and astrocytes in culture.

作者信息

Marchetti D, Stach R W, Saneto R, de Vellis J, Perez-Polo J R

出版信息

Biochem Biophys Res Commun. 1987 Aug 31;147(1):422-7. doi: 10.1016/s0006-291x(87)80138-9.

PMID:2820399

Abstract

The neuronotrophic factor NGF binds to peripheral neurons of the dorsal root ganglion and the sympathetic nervous system. NGF binds to a cell surface receptor, NGFR, on these cells and displays Kd's of 10(-9) and 10(-11)M. NGF receptors have also been reported for basal forebrain magnocellular neurons. In addition, NGF specifically binds to NGFR on Schwann cells although the biological significance of this binding is not known. Here we report that NGF binds in a saturable and specific fashion to receptors on cultured isolated populations of rat astrocytes but not to oligodendrocytes. The binding to astrocytes in culture displayed a Kd of 2.7 +/- 1.0 nM with 36,000 receptors per cell.

摘要

神经营养因子NGF与背根神经节的外周神经元以及交感神经系统结合。NGF与这些细胞表面的一种受体NGFR结合，其解离常数（Kd）分别为10^(-9)M和10^(-11)M。据报道，基底前脑大细胞神经元也存在NGF受体。此外，NGF可特异性地与雪旺细胞上的NGFR结合，但其结合的生物学意义尚不清楚。在此，我们报告NGF以饱和且特异的方式与培养的大鼠星形胶质细胞分离群体上的受体结合，但不与少突胶质细胞结合。在培养物中与星形胶质细胞的结合显示其解离常数为2.7±1.0 nM，每个细胞有36,000个受体。