Otton Letícia Muner, da Silva Campos Marina, Meneghetti Karine Lena, Corção Gertrudes
Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Science, Universidade Federal do Rio Grande do Sul, Sarmento Leite, 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
Arch Microbiol. 2017 Jul;199(5):677-682. doi: 10.1007/s00203-017-1344-7. Epub 2017 Feb 15.
The genus Pseudomonas mainly includes opportunistic pathogens that rely on type IV pili as an important virulence factor, which is associated with adherence and biofilm formation. Pseudomonas infections are well known to be persistent and resilient in nature largely because of the tendency of the species to form biofilms. This study aimed at analyzing environmental strains of Pseudomonas genus with respect to their ability to execute twitching and swarming motilities as well as with respect to their ability to form biofilms both in the presence as well as in the absence of furanone, a substance that has the potential to prevent the formation of biofilms. Strains of Pseudomonas aeruginosa and strains belonging to other species of the genus were analyzed. Twitching and swarming motility assays and biofilm-formation assays, both in the presence as well as in the absence of furanone, were performed. In twitching assay strains belonging to P. aeruginosa outperformed those belonging to other species. Interestingly, it was seen that the presence of furanone had a negative impact on formation of twitching and swarming motility zones. In the case of biofilm assays, it was observed that the presence of furanone resulted in an observable decrease in the degree of adhesion in 30% of the analyzed strains. Thus, from our results, it can be concluded that, as compared to other species, the strains belonging to P. aeruginosa exhibit a higher potential for twitching motility and similar performance in swarming motility and biofilm formation. It can also be concluded that furanone has the potential to interfere with both motilities as well as with biofilm formation.
假单胞菌属主要包括一些机会致病菌，它们依赖IV型菌毛作为重要的毒力因子，这与黏附及生物膜形成有关。假单胞菌感染在本质上以持续性和顽固性著称，很大程度上是因为该菌具有形成生物膜的倾向。本研究旨在分析假单胞菌属的环境菌株在有无呋喃酮（一种具有预防生物膜形成潜力的物质）的情况下进行颤动和群体运动的能力，以及形成生物膜的能力。对铜绿假单胞菌菌株和该属其他种的菌株进行了分析。在有无呋喃酮的情况下分别进行了颤动和群体运动测定以及生物膜形成测定。在颤动测定中，铜绿假单胞菌所属菌株的表现优于其他种的菌株。有趣的是，发现呋喃酮的存在对颤动和群体运动区域的形成有负面影响。在生物膜测定中，观察到呋喃酮的存在导致30%的分析菌株的黏附程度明显降低。因此，从我们的结果可以得出结论，与其他种相比，铜绿假单胞菌所属菌株表现出更高的颤动运动潜力，在群体运动和生物膜形成方面表现相似。还可以得出结论，呋喃酮有可能干扰运动以及生物膜形成。
