固醇结合蛋白的转运分析：用于研究NPC2蛋白细胞内胆固醇转运机制的停流荧光法
Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein.
作者信息
McCauliff Leslie A, Storch Judith
机构信息
Department of Nutritional Sciences and Rutgers Center for Lipid Research, Rutgers University New Brunswick, 65 Dudley Road, New Brunswick, NJ, 08901, USA.
出版信息
Methods Mol Biol. 2017;1583:97-110. doi: 10.1007/978-1-4939-6875-6_9.
Abstract
In this chapter we describe the use of stopped flow fluorescence spectroscopy to analyze the kinetic mechanisms of protein mediated cholesterol transfer to, from, and between model membranes. These assays allow for the detection of protein-membrane interactions that may occur during cholesterol transfer by simply modifying donor or acceptor concentrations, membrane composition, or buffer properties, and analyzing resultant transfer rates.
摘要
在本章中，我们描述了如何使用停流荧光光谱法来分析蛋白质介导的胆固醇在模型膜之间、向模型膜以及从模型膜转移的动力学机制。通过简单地改变供体或受体浓度、膜组成或缓冲液性质，并分析由此产生的转移速率，这些测定方法能够检测胆固醇转移过程中可能发生的蛋白质-膜相互作用。
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本文引用的文献
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2
NPC2, the protein deficient in Niemann-Pick C2 disease, consists of multiple glycoforms that bind a variety of sterols.NPC2是一种在尼曼-匹克C2病中缺乏的蛋白质，由多种能结合多种固醇的糖型组成。
J Biol Chem. 2006 Dec 1;281(48):36710-23. doi: 10.1074/jbc.M608743200. Epub 2006 Oct 2.
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Mechanism of cholesterol transfer from the Niemann-Pick type C2 protein to model membranes supports a role in lysosomal cholesterol transport.胆固醇从尼曼-匹克C2型蛋白转移至模型膜的机制支持其在溶酶体胆固醇转运中的作用。
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Intracellular cholesterol transport.细胞内胆固醇转运
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