Luque J, Delgado M D, Rodriguez-Horche P, Company M T, Pinilla M
Departmento de Bioquimica y Biologia Molecular, Universidad de Alcalã de Henares, Madrid, Spain.
Biosci Rep. 1987 Feb;7(2):113-9. doi: 10.1007/BF01121874.
An increase in bisphosphoglycerate mutase (BPGM) and a decrease in pyruvate kinase (PK), i.e. a decrease in PK/BPGM ratio, was observed in red cell populations from anemic rats containing 95% down to 3% reticulocytes in blood. Such a ratio has been used to study the fractionation of recticulocytes, according to their degree of maturation, after counter-current distribution of those cell populations in dextranpoly (ethylene glycol) two-phase systems. When applying this procedure to the fractionation according to age of erythrocytes from normal rats, the decrease of PK with cellular age was observed without a significant variation in BPGM activity.
在贫血大鼠的红细胞群体中观察到，双磷酸甘油酸变位酶（BPGM）增加，丙酮酸激酶（PK）减少，即PK/BPGM比值降低，这些大鼠血液中网织红细胞含量从95%降至3%。在葡聚糖-聚（乙二醇）双相系统中对这些细胞群体进行逆流分配后，该比值已被用于根据网织红细胞的成熟程度研究其分级分离。当将此方法应用于正常大鼠红细胞按年龄的分级分离时，观察到PK随细胞年龄的降低，而BPGM活性无显著变化。
