Chekanov Konstantin, Feoktistov Alexandr, Lobakova Elena
Department of Bioengineering, Faculty of Biology, Moscow State University, Moscow, Russia.
National Research Nuclear University MEPhi, Centre for Humanities Research and Technology, Moscow, Russia.
Physiol Plant. 2017 Jul;160(3):328-338. doi: 10.1111/ppl.12552. Epub 2017 May 11.
The cephalolichen Peltigera aphthosa (L.) Willd. is characterized by lateral heterogeneity, which manifests itself in the presence of three thallus zones, referred to as the apical, basal and medial zone. These zones differ in terms of interaction between lichen bionts and their physiological activity. The apical thallus zone is more efficient in establishing a contact with cyanobacteria, because of a higher lectin content and a larger overall thallus surface area due to the presence of numerous mycobiont hyphae. Cephalodia are formed in this zone. The interaction between the mycobiont and cyanobiont is more intense in the medial zone. However, the establishment of the contact with cyanobacteria in this zone less probable. The spatial distribution of lectins in the thallus was determined. To reveal the differences in photosynthetic activity in three thallus zones, transient analysis of chlorophyll a fluorescence and the assessment of non-photochemical quenching of excited chlorophyll states were performed. Assimilation of absorbed light energy was more effective in the medial zone. The basal zone was characterized by decreased photosynthetic activity, lichen dissociation and thallus death.
地衣型叶状地衣皮果衣（Peltigera aphthosa (L.) Willd.）具有侧向异质性，表现为存在三个叶状体区域，即顶端区、基部区和中间区。这些区域在地衣共生生物之间的相互作用及其生理活性方面存在差异。顶端叶状体区域与蓝细菌建立接触的效率更高，这是因为凝集素含量较高，并且由于存在大量的真菌共生体菌丝，叶状体的总体表面积更大。在这个区域形成了头状体。真菌共生体和蓝细菌共生体之间的相互作用在中间区更为强烈。然而，在这个区域与蓝细菌建立接触的可能性较小。确定了叶状体中凝集素的空间分布。为了揭示三个叶状体区域光合活性的差异，进行了叶绿素 a 荧光的瞬态分析以及对激发态叶绿素非光化学猝灭的评估。中间区对吸收光能的同化更有效。基部区的特征是光合活性降低、地衣解离和叶状体死亡。
