Shanker G, Pieringer R A
Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Biosci Rep. 1987 Feb;7(2):159-65. doi: 10.1007/BF01121880.
The occurrence and regulation by thyroid hormone of four protein kinases (cyclic AMP independent and dependent, calcium/calmodulin stimulated, and calcium/phosphatidyl serine stimulated protein kinases) was studied in primary cultures of cells dissociated from embryonic mouse brain. Serum from a thyroidectomized calf, which contained low levels of L-3,5,3'-triiodothyronine, T3 (less than 25 ng/100 ml), and thyroxine, T4 (less than 1 microgram/100 ml) was used in the culture medium in place of normal calf-serum (T3, 130 ng/100 ml; T4 5.9 micrograms/100 ml) to render the cultures responsive to exogenously added T3. Cultures grown in hypothyroid calf-serum containing medium had less cAMP dependent and independent protein kinase activity than control cultures grown in normal calf-serum containing medium. However, this activity was restorable to a considerable degree if the cultures grown in hypothyroid calf serum containing medium were supplemented with L-3,5,3'-triiodothyronine (T3). The presence of calcium/calmodulin stimulated protein kinase was also distinctly observed. In comparison, the activity of calcium/phosphatidyl serine stimulated protein kinase was less than the other protein kinases.
在从胚胎小鼠脑部分离的细胞原代培养物中，研究了四种蛋白激酶（环磷酸腺苷非依赖性和依赖性、钙/钙调蛋白刺激型以及钙/磷脂酰丝氨酸刺激型蛋白激酶）的发生情况及其受甲状腺激素的调节。在培养基中使用来自甲状腺切除小牛的血清（其含有低水平的L-3,5,3'-三碘甲状腺原氨酸，T3（小于25 ng/100 ml）和甲状腺素，T4（小于1微克/100 ml））来替代正常小牛血清（T3，130 ng/100 ml；T4 5.9微克/100 ml），以使培养物对外源性添加的T3产生反应。在含有甲状腺功能减退小牛血清的培养基中生长的培养物，其环磷酸腺苷依赖性和非依赖性蛋白激酶活性低于在含有正常小牛血清的培养基中生长的对照培养物。然而，如果在含有甲状腺功能减退小牛血清的培养基中生长的培养物补充L-3,5,3'-三碘甲状腺原氨酸（T3），这种活性在很大程度上是可以恢复的。钙/钙调蛋白刺激型蛋白激酶的存在也清晰可见。相比之下，钙/磷脂酰丝氨酸刺激型蛋白激酶的活性低于其他蛋白激酶。
