环磷胞苷在体外刺激牛乳腺组织的DNA合成。

Cyclic cytidine monophosphate stimulates DNA synthesis by bovine mammary tissue in vitro.

作者信息

Sheffield L G

机构信息

Dairy Science Department, University of Wisconsin, Madison 53706.

出版信息

Cell Biol Int Rep. 1987 Jul;11(7):557-62. doi: 10.1016/0309-1651(87)90018-x.

DOI:10.1016/0309-1651(87)90018-x

PMID:2820591

Abstract

Mammary gland biopsies were taken from midpregnant heifers (n = 4), cut into pieces .5 mm thick and 3 - 5 mm2 and incubated for 48 hours in Eagle's Minimum Essential Medium containing 0, .1 or 1 micrograms/ml insulin and 0, 10(-8), 10(-7), 10(-6), 10(-5), or 10(-4) M dibutyryl cyclic 3', 5', cytidine monophosphate (dbcCMP). With 0 or .1 microgram/ml insulin, dbcCMP decreased incorporation of tritiated thymidine into DNA. Similar declines in DNA synthesis were observed with sodium butyrate, suggesting that the decline was due to the butyrate rather than to a cyclic CMP-specific effect. With 1 micrograms/ml insulin, dbcCMP increased DNA synthesis. Higher levels of dbcCMP reduced DNA synthesis relative to 10(-6)M dbcCMP, as did sodium butyrate. Thus cCMP is capable of stimulating mammary growth.

摘要

从妊娠中期的小母牛（n = 4）获取乳腺活检组织，切成0.5毫米厚、3 - 5平方毫米的小块，并在含有0、0.1或1微克/毫升胰岛素以及0、10^(-8)、10^(-7)、10^(-6)、10^(-5)或10^(-4) M二丁酰环3',5'-胞苷单磷酸（dbcCMP）的伊格尔最低必需培养基中孵育48小时。在含有0或0.1微克/毫升胰岛素的情况下，dbcCMP降低了氚标记胸腺嘧啶核苷掺入DNA的量。用丁酸钠观察到类似的DNA合成下降，表明这种下降是由于丁酸盐而非环CMP特异性效应所致。在含有1微克/毫升胰岛素的情况下，dbcCMP增加了DNA合成。与10^(-6) M dbcCMP相比，更高水平的dbcCMP以及丁酸钠均降低了DNA合成。因此，环CMP能够刺激乳腺生长。