Romero-González Roberto, Liébanas Francisco Javier Arrebola, López-Ruiz Rosalía, Frenich Antonia Garrido
University of Almeria, Analytical Chemistry Area, Department of Chemistry and Physics, Agrifood Campus of International Excellence, Research Centre for Agricultural and Food Biotechnology, Carretera de Sacramento s/n, E-04120 Almeria, Spain.
J AOAC Int. 2016 Nov 1;99(6):1395-1402. doi: 10.5740/jaoacint.16-0273.
The use of high-resolution MS (HRMS) is becoming popular in laboratories for the determination of pesticide residues in food commodities. The recent improvements in the instrumentation have helped to increase the number of active compounds and transformation products that can be monitored within a simple chromatographic run. However, prior to instrumental determination, it is necessary to perform a nonspecific, or generic, sample treatment that allows the efficient extraction of several compounds with very relevant differences in their physical and chemical properties. In this sense, the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and its several modifications and the "dilute-and-shoot" extraction methodology have already revealed an enormous potential for their use together with chromatographic techniques coupled to HRMS. The potentiality and limitations of such a methodological combination have been evaluated in terms of sensitivity and selectivity when they are applied to the analysis of complex food matrixes. An evaluation of the scope of the methods, in terms of efficiency of the extraction and ionization steps, as well as the matrix effect, has also been carried out. Different solutions for the matrix effect have been considered, including improvement in clean-up steps, sample dilution, and matrix compensation by matrix-matched calibration or by the use of isotopically labeled standards.
在食品商品中农药残留量的测定方面，高分辨率质谱（HRMS）在实验室中的应用正变得越来越普遍。仪器设备最近的改进有助于增加在一次简单的色谱运行中可监测的活性化合物和转化产物的数量。然而，在进行仪器测定之前，有必要进行非特异性或通用的样品处理，以便有效地提取几种物理和化学性质差异很大的化合物。从这个意义上讲，快速、简便、廉价、有效、耐用且安全（QuEChERS）方法及其多种改进方法以及“稀释即进样”提取方法，已经显示出它们与与HRMS联用的色谱技术一起使用的巨大潜力。当将这种方法组合应用于复杂食品基质分析时，已从灵敏度和选择性方面评估了其潜力和局限性。还对这些方法在提取和电离步骤的效率以及基质效应方面的适用范围进行了评估。已经考虑了针对基质效应的不同解决方案，包括改进净化步骤、样品稀释以及通过基质匹配校准或使用同位素标记标准进行基质补偿。
