Fouad Rabab, Zachariah Khaled, Khairy Marwa, Khorshied Mervat, Ezzat Wafaa, Sheta Marwa M, Heiba Ahmed
1 Endemic Medicine Department and Hepatology Unit, Faculty of Medicine, Cairo University , Cairo, Egypt .
2 Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University , Cairo, Egypt .
J Interferon Cytokine Res. 2017 Feb;37(2):90-96. doi: 10.1089/jir.2016.0099.
Ribavirin clearly plays a role in chronic hepatitis C treatment response. The equilibrative nucleoside transporter-1 codified by SLC29A1 gene has been associated with ribavirin uptake into hepatocytes and erythrocytes. rs760370A>G single nucleotide polymorphism (SNP) at the SLC29A1 gene may have a role in ribavirin-based regimen treatment response. Accuracy of the polymerase-chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay compared with the TaqMan assay for the detection of the SNP rs760370 at the main ribavirin transporter gene and its relation to sustained virological response in chronic hepatitis C virus (HCV) patients treated with pegylated interferon-ribavirin therapy. The study included 100 chronic HCV patients who were treated with pegylated interferon-ribavirin therapy. The patients were categorized according to the treatment response into responders (50 patients) and null responders (50 patients). rs760370 SNP was measured using TaqMan 5-nuclease assay and by the newly developed PCR-based RFLP assay. The overall accuracy of the newly developed PCR-RFLP assay compared with the TaqMan assay for rs760370 polymorphism detection was 100%. Allelic frequencies at rs760370 were as follows: A/A genotype (28%), A/G genotype (58%), and G/G genotype (14%). Treatment response was not significantly related with rs760370 polymorphism (P = 0.5). Ribavirin-induced anemia was good predictor of sustained virological response (P = 0.001), but was not related to rs760370 polymorphism (P = 0.92). PCR-RFLP assay is an accurate, cost-effective method in the detection of rs760370 compared with TaqMan assay. rs760370 SNP cannot serve as predictor of response in chronic HCV patients treated with interferon ribavirin therapy.
利巴韦林在慢性丙型肝炎治疗反应中显然发挥着作用。由SLC29A1基因编码的平衡核苷转运体-1与利巴韦林进入肝细胞和红细胞的摄取有关。SLC29A1基因的rs760370A>G单核苷酸多态性（SNP）可能在基于利巴韦林的治疗方案反应中起作用。与TaqMan检测法相比，聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测法检测主要利巴韦林转运体基因SNP rs760370的准确性及其与接受聚乙二醇干扰素-利巴韦林治疗的慢性丙型肝炎病毒（HCV）患者持续病毒学应答的关系。该研究纳入了100例接受聚乙二醇干扰素-利巴韦林治疗的慢性HCV患者。根据治疗反应将患者分为应答者（50例）和无应答者（50例）。使用TaqMan 5-核酸酶检测法和新开发的基于PCR的RFLP检测法检测rs760370 SNP。与TaqMan检测法相比，新开发的PCR-RFLP检测法检测rs760370多态性的总体准确性为100%。rs760370的等位基因频率如下：A/A基因型（28%）、A/G基因型（58%）和G/G基因型（14%）。治疗反应与rs760370多态性无显著相关性（P = 0.5）。利巴韦林诱导的贫血是持续病毒学应答的良好预测指标（P = 0.001），但与rs760370多态性无关（P = 0.92）。与TaqMan检测法相比，PCR-RFLP检测法是检测rs760370的一种准确、经济有效的方法。rs760370 SNP不能作为接受干扰素利巴韦林治疗的慢性HCV患者反应的预测指标。
