Silverman J A, Benson S A
Department of Molecular Biology, Princeton University, New Jersey 08544.
J Bacteriol. 1987 Oct;169(10):4830-3. doi: 10.1128/jb.169.10.4830-4833.1987.
Mutations which prevent absorption of the bacteriophage K20 to Escherichia coli K-12 were selected by using an altered OmpF protein which confers the ability to grow on maltodextrin in the absence of the LamB maltoporin. The mutations map in the rfa gene cluster and alter the structure of the lipopolysaccharide core.
通过使用一种改变的OmpF蛋白来筛选阻止噬菌体K20吸附到大肠杆菌K-12上的突变,该蛋白赋予了在没有LamB麦芽糖孔蛋白的情况下利用麦芽糖糊精生长的能力。这些突变定位在rfa基因簇中,并改变了脂多糖核心的结构。