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银染聚丙烯酰胺凝胶中沙门氏菌脂多糖化学型之间的形态异质性。

Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.

作者信息

Hitchcock P J, Brown T M

出版信息

J Bacteriol. 1983 Apr;154(1):269-77. doi: 10.1128/jb.154.1.269-277.1983.

DOI:10.1128/jb.154.1.269-277.1983
PMID:6187729
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217456/
Abstract

The morphological heterogeneity of lipopolysaccharides (LPSs) among salmonella mutants with different LPS chemotypes was analyzed in silver-stained polyacrylamide gels. The biochemical differences in the LPS chemotypes were reflected in the unique profiles of the purified LPSs. The LPS profiles in the whole-cell lysates were also unique for each chemotype. (Whole-cell lysates were assessed by a method which preferentially silver stains LPS and by a proteinase K digest of whole-cell lysates. The silver-stained LPS profiles of proteinase K-digested lysates were similar to the homologous purified LPS and could be used to preliminarily characterize the LPS chemotype before purification.) In summary, biochemical variation in LPS composition can be detected in silver-stained polyacrylamide gels.

摘要

在银染聚丙烯酰胺凝胶中分析了具有不同脂多糖（LPS）化学型的沙门氏菌突变体中LPS的形态异质性。LPS化学型的生化差异反映在纯化LPS的独特图谱中。每种化学型的全细胞裂解物中的LPS图谱也具有独特性。（通过优先对LPS进行银染的方法以及对全细胞裂解物进行蛋白酶K消化来评估全细胞裂解物。蛋白酶K消化的裂解物的银染LPS图谱与同源纯化LPS相似，可用于在纯化前初步表征LPS化学型。）总之，在银染聚丙烯酰胺凝胶中可以检测到LPS组成的生化变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/5730fe6650d7/jbacter00245-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/253e28fefa49/jbacter00245-0281-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/97662789dc99/jbacter00245-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/bec0917b4aa3/jbacter00245-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/5730fe6650d7/jbacter00245-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/253e28fefa49/jbacter00245-0281-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/97662789dc99/jbacter00245-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/bec0917b4aa3/jbacter00245-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/217456/5730fe6650d7/jbacter00245-0285-a.jpg

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