Richmond R, Turner N C, Maltby N, Heavey D, Vial J, Dollery C T, Taylor G W
Department of Clinical Pharmacology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
J Chromatogr. 1987 Jul 3;417(2):241-51. doi: 10.1016/0378-4347(87)80117-2.
A rapid and simple method for the on-line concentration and high-performance liquid chromatographic (HPLC) purification of the leukotrienes in good yield from biological fluids is described. Readily available antisera are used in conjunction with this system to give a specific and sensitive assay for leukotrienes B4, C4 and D4 with sub-nanogram limits of detection. Tritium-labelled leukotrienes are used as internal standards, both to locate the leukotrienes post-HPLC and to accurately determine recoveries.
本文描述了一种从生物流体中高效在线浓缩和通过高效液相色谱（HPLC）纯化白三烯的快速简便方法。该系统结合了易于获得的抗血清，可对白三烯B4、C4和D4进行特异性灵敏检测，检测限低至亚纳克。用氚标记的白三烯作为内标，既用于HPLC后定位白三烯，又用于准确测定回收率。
