Tseng L, Malbon C C, Lane B, Kaplan C, Mazella J, Dahler H, Tseng A
Department of Obstetrics/Gynecology, State University of New York, Stony Brook 11794.
Hum Reprod. 1987 Jul;2(5):371-7. doi: 10.1093/oxfordjournals.humrep.a136553.
We have previously demonstrated that the aromatase activity in human endometrial stromal cells is stimulated by progestin and enhanced by oestrogen. In this study, we have investigated the effect of forskolin (Fk), an agent that stimulates the hormone-sensitive adenylate cyclase in mammalian cells, on the intracellular cAMP content and aromatase activity in endometrial stromal cells in primary culture. Stromal cells were isolated from proliferative and secretory endometria and were individually cultured in nutrient medium or medium supplemented with medroxyprogesterone acetate (MPA), oestradiol (E2) and Fk, separately or in combination. The intracellular cAMP content of stromal cells was increased after incubation with Fk. Stromal cells treated with Fk alone or FK and MPA for 1-3 days sustained the elevated intracellular cAMP content but 90% of this nucleotide was released to the medium. Aromatase activity was either not affected or was increased up to 5-fold over the control by Fk alone. Forskolin exerted a synergistic effect upon aromatase activity in the presence of progestin or progestin and oestradiol. Sequential incubation of the stromal cells with MPA and then Fk indicated that the additional increase in aromatase activity caused by Fk occurred after 24 h of incubation. These results demonstrate that intracellular cAMP exerts a stimulatory effect on aromatase activity in progestin-conditioned stromal cells. We also investigated whether the endometrial aromatase activity changes in vivo during the reproductive cycle by measuring the activities in endometrial specimens obtained from women during their reproductive age.(ABSTRACT TRUNCATED AT 250 WORDS)
我们之前已经证明，人子宫内膜基质细胞中的芳香化酶活性受孕激素刺激，并被雌激素增强。在本研究中，我们研究了福斯高林（Fk）（一种刺激哺乳动物细胞中激素敏感性腺苷酸环化酶的试剂）对原代培养的子宫内膜基质细胞内cAMP含量和芳香化酶活性的影响。从增殖期和分泌期子宫内膜中分离出基质细胞，并分别在营养培养基或补充有醋酸甲羟孕酮（MPA）、雌二醇（E2）和Fk的培养基中单独或联合培养。与Fk孵育后，基质细胞内的cAMP含量增加。单独用Fk或Fk与MPA处理基质细胞1 - 3天，细胞内cAMP含量持续升高，但其中90%的核苷酸释放到培养基中。单独使用Fk时，芳香化酶活性要么不受影响，要么比对照增加高达5倍。在存在孕激素或孕激素与雌二醇的情况下，福斯高林对芳香化酶活性发挥协同作用。基质细胞先后用MPA和Fk孵育表明，Fk引起的芳香化酶活性额外增加在孵育24小时后出现。这些结果表明，细胞内cAMP对孕激素预处理的基质细胞中的芳香化酶活性具有刺激作用。我们还通过测量育龄期妇女子宫内膜标本中的活性，研究了生殖周期中体内子宫内膜芳香化酶活性是否发生变化。（摘要截短至250字）
