Davidson E, Daldal F
Cold Spring Harbor Laboratory, NY 11724.
J Mol Biol. 1987 May 5;195(1):25-9. doi: 10.1016/0022-2836(87)90324-x.
Detailed comparison of the 'Rhodopseudomonas sphaeroides GA' strain used by Gabellini et al. (1985) with genuine R. sphaeroides and R. capsulata strains indicated that the previously reported fbc operon of R. sphaeroides (Gabellini and Sebald, 1986) encoding the structural genes for the Rieske Fe-S protein, cytochrome b and cytochrome c1 subunits of the ubiquinol:cytochrome c2 oxidoreductase, is not from R. sphaeroides, but is rather from a strain of R. capsulata. Consequently, the genuine bc1 genes from R. sphaeroides were cloned using corresponding R. capsulata genes as probes, and a partial nucleotide sequence for the Rieske Fe-S protein of R. sphaeroides was determined and compared with that of R. capsulata.
Gabellini等人(1985年)使用的“球形红假单胞菌GA”菌株与真正的球形红假单胞菌和荚膜红假单胞菌菌株的详细比较表明,先前报道的球形红假单胞菌fbc操纵子(Gabellini和Sebald,1986年)编码泛醇:细胞色素c2氧化还原酶的 Rieske Fe-S蛋白、细胞色素b和细胞色素c1亚基的结构基因,并非来自球形红假单胞菌,而是来自荚膜红假单胞菌的一个菌株。因此,以相应的荚膜红假单胞菌基因作为探针克隆了球形红假单胞菌的真正bc1基因,并测定了球形红假单胞菌 Rieske Fe-S蛋白的部分核苷酸序列,并与荚膜红假单胞菌的进行了比较。