[Reduced restriction of EcoK in the presence of the plasmid pKM ard+. II. Cloning of the gene ard].

Plasmid pKM101 affects the I type restriction EcoK in E. coli. The gene ard responsible for alleviation of EcoK restriction was shown to be located within the BglIIB fragment of pKM101. Here we have cloned ard gene into high copy vectors pBR322 and pUC12. Plasmid pD12 was constructed by introducing into pUC12 a 1.87 kb HindIII-Pst fragment, carrying ard gene. Tn5 and Tn1000 insertions were obtained in the ard gene region.