Shakya Viplendra P S, Idnurm Alexander
Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, USA; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, USA.
Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, USA; School of BioSciences, University of Melbourne, Australia.
Fungal Genet Biol. 2017 Apr;101:20-30. doi: 10.1016/j.fgb.2017.02.005. Epub 2017 Feb 16.
Light is an environmental signal that influences reproduction in the Mucoromycotina fungi, as it does in many other species of fungi. Mating in Phycomyces blakesleeanus is inhibited by light, but the molecular mechanisms for this inhibition are uncharacterized. In this analysis, the role of the light-sensing MadA-MadB complex in mating was tested. The MadA-MadB complex is homologous to the Neurospora crassa White Collar complex. Three genes required for cell type determination in the sex locus or pheromone biosynthesis are transcriptionally-regulated by light and are controlled by MadA and MadB. This regulation acts through the plus partner, indicating that the inhibitory effect of light on mating is executed through only one of the two sexes. These results are an example whereby the mating types of fungi have acquired sex-specific properties beyond their role in conferring cell-type identity, and provide insight into how sex-determining chromosomal regions can expand the traits they control.
光作为一种环境信号，会影响毛霉亚门真菌的繁殖，在许多其他真菌物种中也是如此。布氏梨形孢的交配受光抑制，但其抑制的分子机制尚未明确。在本分析中，对光感应MadA-MadB复合物在交配中的作用进行了测试。MadA-MadB复合物与粗糙脉孢菌的白领复合物同源。性别位点或信息素生物合成中细胞类型确定所需的三个基因受光转录调控，并由MadA和MadB控制。这种调控通过正交配型起作用，表明光对交配的抑制作用仅通过两性中的一方来执行。这些结果表明，真菌的交配型除了在赋予细胞类型身份方面发挥作用外，还具有性别特异性特性，并为性别决定染色体区域如何扩展其控制的性状提供了见解。
