College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Biology and Soft Matter Division, Oak Ridge National Laboratory, TN 37830, USA.
Bioresour Technol. 2017 May;232:113-118. doi: 10.1016/j.biortech.2017.02.014. Epub 2017 Feb 8.
A systematic study was performed to understand interactions among biomass loading during ionic liquid (IL) pretreatment, biomass type and biomass structures. White poplar and eucalyptus samples were pretreated using 1-ethyl-3-methylimidazolium acetate (EmimOAc) at 110°C for 3h at biomass loadings of 5, 10, 15, 20 and 25wt%. All of the samples were chemically characterized and tested for enzymatic hydrolysis. Physical structures including biomass crystallinity and porosity were measured by X-ray diffraction (XRD) and small angle neutron scattering (SANS), respectively. SANS detected pores of radii ranging from ∼25 to 625Å, enabling assessment of contributions of pores with different sizes to increased porosity after pretreatment. Contrasting dependences of sugar conversion on white poplar and eucalyptus as a function of biomass loading were observed and cellulose crystalline structure was found to play an important role.
进行了一项系统研究，以了解生物质负载在离子液体（IL）预处理、生物质类型和生物质结构之间的相互作用。采用 1-乙基-3-甲基咪唑醋酸盐（EmimOAc）在 110°C 下对白榆和桉树样品进行预处理，生物质负载分别为 5wt%、10wt%、15wt%、20wt%和 25wt%。对所有样品进行了化学表征，并进行了酶水解测试。通过 X 射线衍射（XRD）和小角中子散射（SANS）分别测量了物理结构，包括生物质结晶度和孔隙率。SANS 检测到半径范围从 25 到 625Å 的孔，这使得可以评估预处理后不同大小的孔对增加孔隙率的贡献。观察到糖转化率对白榆和桉树的依赖性随生物质负载的变化而变化，并且发现纤维素结晶结构起着重要作用。
