Shi X L, Fu Y Q, Abdullahi A Y, Wang M W, Yang F, Yu X G, Pan W D, Yan X X, Hang J X, Zhang P, Li G Q
College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong Province,510642,People's Republic of China.
J Helminthol. 2018 Jan;92(1):22-33. doi: 10.1017/S0022149X17000116. Epub 2017 Feb 20.
Ancylostoma tubaeforme may infect canids, felids and humans, and pose a potential risk to public health. Polymerase chain reaction (PCR) techniques were used to amplify the complete mitochondrial (mt) genome sequence of A. tubaeforme from cats and to analyse its sequence characteristics after molecular identification based on the internal transcribed spacer ITS1+ sequence. The results show that the complete mt genome sequence (GenBank accession number KY070315) of A. tubaeforme from cats was 13,730 bp in length, including 12 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, two non-coding regions and an AT-rich region. The nucleotide content of A and T was 77.93%, biased toward A and T. Twelve protein-coding genes used ATT, TTG and GTG as initiation codons, and TAA, TAG, TA and T as termination codons. The length of the 22 tRNA genes ranged from 52 to 62 bp, their predicted secondary structures were D loops and V loops. The lengths of the two rRNAs were 958 and 697 bp. Phylogenetic analyses showed that A. tubaeforme from cats was in the lineage of Ancylostoma, having a close phylogenetic relationship with A. caninum. This study reports for the first time the mt genome of A. tubaeforme from cats in China, which could enhance the mt genome database of Ancylostomatidae nematodes, and it offers the scientific basis for further studies in the genetic diversity of hookworms among different hosts.
管形钩口线虫可感染犬科动物、猫科动物和人类，对公众健康构成潜在风险。采用聚合酶链反应（PCR）技术扩增猫体内管形钩口线虫的完整线粒体（mt）基因组序列，并基于转录间隔区ITS1 +序列进行分子鉴定后分析其序列特征。结果显示，猫源管形钩口线虫的完整mt基因组序列（GenBank登录号KY070315）长度为13,730 bp，包括12个蛋白质编码基因、22个转运RNA（tRNA）基因、2个核糖体RNA（rRNA）基因、2个非编码区和1个富含AT的区域。A和T的核苷酸含量为77.93%，偏向于A和T。12个蛋白质编码基因使用ATT、TTG和GTG作为起始密码子，使用TAA、TAG、TA和T作为终止密码子。22个tRNA基因的长度在52至62 bp之间，其预测的二级结构为D环和V环。两个rRNA的长度分别为958和697 bp。系统发育分析表明，猫源管形钩口线虫属于钩口线虫属谱系，与犬钩口线虫具有密切的系统发育关系。本研究首次报道了中国猫源管形钩口线虫的mt基因组，这可以丰富钩口科线虫的mt基因组数据库，并为进一步研究不同宿主中钩虫的遗传多样性提供科学依据。
