Zavala-Vega Sergio, Castro-Escarpulli Graciela, Hernández-Santos Hector, Salinas-Lara Citlatepetl, Palma Icela, Mejía-Aranguré Juan Manuel, Gelista-Herrera Noemí, Rembao-Bojorquez Daniel, Ochoa Sara A, Cruz-Córdova Ariadnna, Xicohtencatl-Cortes Juan, Uribe-Gutiérrez Gabriel, Arellano-Galindo José
Área de Virología, Laboratorio de Infectología, Hospital Infantil de México Federico Gómez, Mexico; Laboratorio de Bacteriología Médica, Departamento de Microbiología y Programa de Doctorado en Ciencias en Biomedicina y Biotecnología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico; Departamento de Neuropatología, Instituto Nacional de Neurología Manuel Velazco Suárez, Mexico.
Laboratorio de Bacteriología Médica, Departamento de Microbiología y Programa de Doctorado en Ciencias en Biomedicina y Biotecnología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico.
Pathol Res Pract. 2017 Mar;213(3):271-276. doi: 10.1016/j.prp.2016.12.006. Epub 2016 Dec 24.
Several risk factors are involved in glioblastoma, including cytomegalovirus (CMV). This research was carried out to determine the rate of CMV infection, as well as HSV 1/2 and EBV in brain tissue, in patients with glioblastomamultiforme (GBM). The tissues were tested using immunohistochemistry, PCR, in situ hybridization and real-time PCR. At least, one HHV was detected in 21/29 (72%) patients as follows: single infections with HSV-1/2 in 4/21 (19%), EBV in 6/21 (28.6%) and CMV in 1/21 (4.8%). Mixed viral infection, HSV-1/2 and EBV were detected in 4/21 patients (19%), CMV and EBV in 5/21 (23.8%), and HSV-1/2, EBV, and CMV in 1/21. The CMV viral load ranged from 3×10 to 4.33×10 genome/100ng of tissue. Genotype based on CMV gB was 3/7 where 2/3 was gB1 and 1/3 gB4. HSV, EBV and CMV were frequently found in brain tissues, more in mix in a population reported as highly seropositive.
胶质母细胞瘤涉及多种风险因素，包括巨细胞病毒（CMV）。本研究旨在确定多形性胶质母细胞瘤（GBM）患者脑组织中CMV感染率以及HSV 1/2和EBV的感染率。使用免疫组织化学、聚合酶链反应（PCR）、原位杂交和实时PCR对组织进行检测。在21/29（72%）的患者中至少检测到一种人类疱疹病毒（HHV），情况如下：4/21（19%）患者为HSV-1/2单一感染，6/21（28.6%）患者为EBV单一感染，1/21（4.8%）患者为CMV单一感染。4/21（19%）患者检测到HSV-1/2和EBV混合病毒感染，5/21（23.8%）患者检测到CMV和EBV混合感染，1/21患者检测到HSV-1/2、EBV和CMV混合感染。CMV病毒载量范围为3×10至4.33×10基因组/100ng组织。基于CMV gB的基因型为3/7，其中2/3为gB1，1/3为gB4。HSV、EBV和CMV在脑组织中经常被发现，在一个血清学阳性率很高的人群中混合感染更为常见。
