Yilmaz Semih, Azizoglu Ugur, Ayvaz Abdurrahman, Temizgul Ridvan, Atciyurt Zehra Büşra, Karabörklü Salih
Department of Agricultural Biotechnology, Erciyes University, Kayseri, Turkey.
Department of Crop and Animal Production, Erciyes University, Tomarza Mustafa Akıncıoğlu Vocational College, Kayseri, Turkey.
Microb Pathog. 2017 Apr;105:81-85. doi: 10.1016/j.micpath.2017.02.016. Epub 2017 Feb 13.
Bacillus thuringiensis (Berliner) (Bt) is well known for having toxicity against pest insects because of their ability to form endospores and broad-range activity of their parasporal inclusions. In this study, a new member of cry2A gene from previously characterized native B. thuringiensis SY49-1 strain was cloned, expressed and used for its activity against Culex pipiens (Diptera: Culicidae) larvae. The sequence analysis of the cloned cry2A gene revealed that it encodes a polypeptide of 633 aa residues with 99% identity to Cry2Aa protein with expected molecular weight of 70.7 kDa. Bacillus thuringiensis delta-endotoxin nomenclature committee designed our sequence as Cry2Aa18 being a new member of Bt toxins. Bioassays against last instar larvae of C. pipiens indicated that Cry2Aa18 has considerable toxicity with LC of 630 μg ml. In order to prevent the spread of infectious diseases mediated by C. pipiens, this newly characterized cry2Aa18 gene could constitute as an important biological control tool for controlling mosquito larvae living in freshwater systems and can be used as a good alternative for minimizing the use of chemicals.
苏云金芽孢杆菌（Berliner）（Bt）以其形成内生孢子的能力及其伴孢晶体的广泛活性而对害虫具有毒性而闻名。在本研究中，从先前鉴定的苏云金芽孢杆菌SY49-1菌株中克隆、表达了cry2A基因的一个新成员，并用于检测其对致倦库蚊（双翅目：蚊科）幼虫的活性。对克隆的cry2A基因的序列分析表明，它编码一个由633个氨基酸残基组成的多肽，与Cry2Aa蛋白具有99%的同一性，预期分子量为70.7 kDa。苏云金芽孢杆菌δ-内毒素命名委员会将我们的序列设计为Cry2Aa18，它是Bt毒素的一个新成员。对致倦库蚊末龄幼虫的生物测定表明，Cry2Aa18具有相当大的毒性，LC为630 μg/ml。为了防止由致倦库蚊介导的传染病传播，这个新鉴定的cry2Aa18基因可作为控制生活在淡水系统中的蚊虫幼虫的重要生物防治工具，并可作为减少化学药剂使用的良好替代品。
