Zheng Yadong, Guo Xiaola, Su Meng, Guo Aijiang, Ding Juntao, Yang Jing, Xiang Haitao, Cao Xiaoan, Zhang Shaohua, Ayaz Mazhar, Luo Xuenong
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China.
Vet Parasitol. 2017 Feb 15;235:29-36. doi: 10.1016/j.vetpar.2017.01.012. Epub 2017 Jan 15.
Extracellular vesicles (EVs) play a role in intercellular communications via exchanging biological molecules, being involved in host-parasite interplay. Little is to date known about E. multilocularis EVs and their biological activities. Here spherical EVs secreted by E. multilocularis metacestodes were shown to range predominately from 34nm to 95nm in diameter. A total of 433 proteins were identified in the EVs, and the proteins involved in binding (42%) and catalytic activity (41%) were most frequently represented. Moreover, the proteins associated with EV biogenesis and trafficking, including annexin, 14-3-3, tetraspanin and heat shock protein 70kDa, were highly enriched. It was shown that the EVs remarkably suppressed NO produced by activated RAW macrophages via downregulation of inducible nitric oxide synthase expression (p <0.01). Suppression of pro-inflammatory cytokines, especially IL-1α and IL-1β, was also observed post treatment with the EVs. Conversely, increased expression of the majority (10/11) of key components involved in the LPS/TLR4 pathway was induced by the EVs. These results demonstrate a regulatory effect of E. multilocularis EVs on macrophages, suggesting a role in parasite-host interactions.
细胞外囊泡（EVs）通过交换生物分子在细胞间通讯中发挥作用，参与宿主与寄生虫的相互作用。迄今为止，关于多房棘球绦虫EVs及其生物学活性知之甚少。在此，多房棘球绦虫成虫囊泡分泌的球形EVs直径主要在34nm至95nm之间。在这些EVs中总共鉴定出433种蛋白质，其中参与结合（42%）和催化活性（41%）的蛋白质最为常见。此外，与EV生物发生和运输相关的蛋白质，包括膜联蛋白、14-3-3、四跨膜蛋白和70kDa热休克蛋白，高度富集。结果表明，EVs通过下调诱导型一氧化氮合酶的表达显著抑制活化的RAW巨噬细胞产生的NO（p<0.01）。用EVs处理后，还观察到促炎细胞因子，尤其是IL-1α和IL-1β的表达受到抑制。相反，EVs诱导LPS/TLR4途径中大多数（10/11）关键成分的表达增加。这些结果证明了多房棘球绦虫EVs对巨噬细胞的调节作用，表明其在寄生虫与宿主相互作用中发挥作用。
