Yan Wenkai, Xiao Xiang, Zhang Yu
State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Institute of Oceanology, Shanghai Jiao Tong University, Shanghai 200240, China.
J Biotechnol. 2017 Mar 20;246:33-35. doi: 10.1016/j.jbiotec.2017.02.016. Epub 2017 Feb 16.
Microbiologically induced calcium carbonate precipitation shows the potential for use in bioremediation and construction consolidation, but the efficiency of this process must be improved. Lysinibacillus sphaericus LMG 22257 is a gram-positive ureolytic strain that has recently been applied for consolidating construction by mediating calcium carbonate precipitation. The complete genome sequence of L. sphaericus LMG 22257 is 3,436,578 base pairs with a GC content of 38.99%. The urea degradation pathway and genes related to extracellular polymeric substance biosynthesis were also identified. The strain can tolerate high alkalinity (pH up to 10) and high urea concentration (up to 3M). These findings provide insights into the microbiologically induced carbonate precipitation and extend the application of the metabolic potential of L. sphaericus LMG 22257 for bioremediation.
微生物诱导碳酸钙沉淀显示出在生物修复和建筑加固方面的应用潜力，但该过程的效率必须提高。球形赖氨酸芽孢杆菌LMG 22257是一种革兰氏阳性尿素分解菌株，最近已被应用于通过介导碳酸钙沉淀来加固建筑。球形赖氨酸芽孢杆菌LMG 22257的完整基因组序列为3436578个碱基对，GC含量为38.99%。还鉴定了尿素降解途径和与细胞外聚合物生物合成相关的基因。该菌株能够耐受高碱度（pH值高达10）和高尿素浓度（高达3M）。这些发现为微生物诱导的碳酸盐沉淀提供了见解，并扩展了球形赖氨酸芽孢杆菌LMG 22257在生物修复方面的代谢潜力应用。
