脐血可替宁和谷胱甘肽-S-转移酶基因多态性对出生结局的影响。
The Impacts of Cord Blood Cotinine and Glutathione-S-Transferase Gene Polymorphisms on Birth Outcome.
作者信息
Huang Kuan-Han, Chou An-Kuo, Jeng Suh-Fang, Ng Sharon, Hsieh Chia-Jung, Chen Mei-Huei, Chen Pau-Chung, Hsieh Wu-Shiun
机构信息
Department of Pediatrics, National Taiwan University Children's Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan; Department of Pediatrics, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan.
Department of Pediatrics, National Taiwan University Hospital, Hsinchu Branch, Hsinchu, Taiwan.
出版信息
Pediatr Neonatol. 2017 Aug;58(4):362-369. doi: 10.1016/j.pedneo.2016.08.006. Epub 2017 Jan 17.
BACKGROUND
This study aimed to investigate the association between cord blood cotinine levels and birth outcome, and to determine whether fetal metabolic gene polymorphisms of glutathione-S-transferase (GST) modulate the effect of environmental tobacco smoke exposure during pregnancy.
METHODS
This study included 328 maternal and neonatal pairs. Maternal and cord blood cotinine levels were measured using high performance liquid chromatography. The GST T1 (GSTT1) and GST M1 (GSTM1) polymorphisms were examined using the polymerase chain reaction method. The birth outcomes included birth weight, length, and head circumference, and the risks of having low birth weight and being small for gestational age (SGA).
RESULTS
Cord cotinine level had a dose-dependent impact on the reduction of birth weight, length, and head circumference in newborns. Elevation of cord blood cotinine concentration increased the rate of SGA and low birth weight. The neonates who had GSTT1 or GSTM1 polymorphism were associated with an increased risk of being SGA. A combination of the GSTT1 and GSTM1 null genotype exacerbated the effect of maternal environmental tobacco smoke exposure on SGA more than the presence of either genotype alone (odds ratio=8.90, 95% confidence interval=1.00-79.5).
CONCLUSION
Cord blood cotinine adversely affects birth outcomes. GSTT1 and GSTM1 null genotype may modify the effect of cord blood cotinine on birth outcomes.
背景
本研究旨在调查脐血可替宁水平与出生结局之间的关联，并确定谷胱甘肽 - S - 转移酶（GST）的胎儿代谢基因多态性是否会调节孕期环境烟草烟雾暴露的影响。
方法
本研究纳入了328对母婴。采用高效液相色谱法测量母体和脐血中的可替宁水平。使用聚合酶链反应法检测GST T1（GSTT1）和GST M1（GSTM1）多态性。出生结局包括出生体重、身长和头围，以及低出生体重和小于胎龄儿（SGA）的风险。
结果
脐血可替宁水平对新生儿出生体重、身长和头围的降低有剂量依赖性影响。脐血可替宁浓度升高会增加SGA和低出生体重的发生率。具有GSTT1或GSTM1多态性的新生儿患SGA的风险增加。与单独存在任何一种基因型相比，GSTT1和GSTM1无效基因型的组合更能加剧母体环境烟草烟雾暴露对SGA的影响（优势比 = 8.90，95%置信区间 = 1.00 - 79.5）。
结论
脐血可替宁对出生结局有不利影响。GSTT1和GSTM1无效基因型可能会改变脐血可替宁对出生结局的影响。
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